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Dissertations |
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PRISCILA BEZERRA DE LIMA
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ASSESSMENT OF CARDIOPULMONARY FUNCTION OF PATIENTS SURVIVING COVID-19 IN OUTDOOR REHABILITATION
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Advisor : MAXWELL BARBOSA DE SANTANA
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COMMITTEE MEMBERS :
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EDGARD MORYA
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MAXWELL BARBOSA DE SANTANA
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RODRIGO LUIS FERREIRA DA SILVA
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TANIA MARA PIRES MORAES
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Data: Apr 12, 2022
Ata de defesa assinada:
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Show Abstract
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COVID-19 is a disease that broke out in late December 2019 in China and became an unprecedented pandemic in March 2020 to the present day. There is concern that COVID-19 is a disease that causes impairment, mainly, of the functions of the cardiorespiratory system and, according to the clinical severity presented, there may be a deficit in respiratory muscle function and exercise tolerance, causing limitations that include difficulties in performing basic daily activities. Objective: to evaluate the cardiopulmonary function of COVID-19 survivors undergoing outpatient rehabilitation in the city of Santarém. Material and Method: After submission and approval by the Research Ethics Committee with human beings, an analytical-descriptive, longitudinal prospective, quantitative and clinical interventional interference study was carried out. An evaluation was carried out consisting of: interview through a pre-established questionnaire; pulmonary function test through spirometry; six-minute step test (TD6), in addition to dyspnea (Medical Research Council - MRC) and perceived exertion (Borg Scale) scales. Statistical analyzes were performed using Microsoft Excel 2010 software and R software. Results and Discussion: The sample consisted of 30 volunteers affected by COVID-19 undergoing outpatient rehabilitation, where males were prevalent (73%), the average age was 53 ± 13 years, with a predominant age group from 56 to 75 years, 60% had the disease in its severe form. Compensatedly controlled comorbidities were found not to be risk factors for disease severity. Just as there was no relationship between age and gender with the outcome of the disease, which suggests that anyone is predisposed to COVID-19 infection. The first symptoms of the disease did not define nor were related to the hospitalization history, making the patient's stability during the course of the disease unpredictable. On the other hand, pulmonary impairment through chest tomography and hospitalization history generated a strong impact with the ventilatory support used, where respiratory support therapy and hospitalization time were directly related to the severity of the disease. Spirometry as an evaluative criterion for outpatient rehabilitation showed lower than normal reference values after COVID-19, evidencing restrictive disorders. Functionality scales after outpatient rehabilitation showed a mild degree of MRC, changes in cognitive behavior in category 6 of the MIF. When evaluating the TD6, variables such as age, sex and ∆WF explain, in part, the number of steps climbed and, generally, they are predictors for physical capacity tests in other physical capacity tests. And as expected, the decrease in TD6 performance with aging is strongly associated, since with advancing age there is a reduction in physical capacity. Final considerations: The patients in this study are the survivors of the first two strong waves of the disease. In the current scenario, even with the availability of mass vaccination, with a significant reduction in the number of cases, hospitalizations and deaths, the third wave has already been experienced. Therefore, it is necessary to maintain a state of alert in order to avoid the worsening of this serious disease and to develop more and more studies to contain it, including to welcome the survivors of the first waves, as the premise of the postnatal syndrome is increasingly strengthened. COVID-19, causing numerous functional limitations and long-term public health disruptions. It is essential, in a good cardiopulmonary evaluation, the inclusion and association of physical capacity tests with pulmonary function tests, in order to maintain the follow-up of these patients as well as measure the functional impact over time after acute infection of the illness.
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2
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GABRIELA DOS SANTOS RODRIGUES
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Combined approach of inverse virtual screening and ligand-based virtual screening for the identification of protein targets for aphidicolin and new hits against Leishmania major
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Advisor : GABRIELA BIANCHI DOS SANTOS
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COMMITTEE MEMBERS :
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RYAN DA SILVA RAMOS
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GLAUBER VILHENA DA COSTA
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GABRIELA BIANCHI DOS SANTOS
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KELLY CHRISTINA FERREIRA CASTRO
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Data: Apr 29, 2022
Ata de defesa assinada:
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Show Abstract
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Leishmaniasis are a group of diseases caused by more than 20 species of protozoa of the genus Leishmania, prevalent in tropical and developing countries. The existing pharmacological treatment presents serious limitations such as toxicity and parasite resistance, therefore, the search for new therapeutic agents is necessary. Molecular modeling methods are useful to facilitate the design of safer and more efficient antiparasitic drugs, especially from natural sources. There are several bioactive natural products in the literature against Leishmania spp. With unknown mechanism of action, including aphidicolin, a selective inhibitor of viral and human DNA polymerase-α, produced by the fungi Cephalosporium aphidicola and Nigrospora sphaerica. Hence, this work aimed to find potential biological targets of aphidicolin and derivatives through virtual reverse screening. We used Protein Data Bank (PDB) for the search ofL. major targets. The targets selected for molecular docking on the DockThor server are those whose ligands showed steric and electronic similarity >0.5 to aphidicolin and RMSD values< 2 Å. Thus, we considered N-myristoyltransferase (NMT), methionyl t-RNA synthetase (MetRS) and map-kinase(MAPK) as possible targets of a phidicolans. Considering the poor pharmacokinetic and physicochemical properties of aphidicolin and derivatives, we used PharmGist server to align the molecules and to build a pharmacophoric model. We evaluated the model by hierarchical cluster analysis (HCA) and Pearson's correlation in Minitab software. Due to the number of hydrophobic properties, we built a pharmacophoric model for each target and we subjected to virtual screening on the Pharmit server. After that, we filtered the new hits through drug-similar property calculation in Osiris Data-Warrior software, as well toxicity alerts in Derek software and pharmacokinetics on PreADMETweb server. We used SissADME web server to predict water solubility and synthetic accessibility. In the end, we selected only 02 molecules for NMT, 09 for MetRS (02 in common with NMT) and none for MAPK. In order to evaluate a potential dual mechanism, wedocked these molecules in NTM, MetRS and MAPK using DockThor server. Statistical analyzes of the 07 best valuesof binding affinity (∆G) of each complex were performed in the GraphPad Prism software using the ANOVA-one way and ANOVA-two way tests. The results showed that the molecules MP-002-507-460, MP-002-528-375 and MP-002-911-105 exhibited ∆G values significantly comparable to the co-crystallized ligands and superior to miltefosine, used as a negative control. However, we observed considerable selectivity for NMT. After analyzing the interactions in the Disco-very Studio software, only MP-002-507-460 and MP-002-911-105 showed interaction patterns more similar to the positive control. The prediction of biological activity through the PASS server revealed that the two molecules, steroid analogues, exhibited a moderate probability of acting as leishmanicidal agents. Thus, the two molecules found through virtual screening are promising candidates for in vitro assays on LmNMT models to validate the theoretical results presented in this work.
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3
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KARINA ALCÂNTARA DE SOUSA
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Volatile constituents and anticholinesterase activity of the essential oil of three chemotypes of Lippia alba (Mill.) N.E.Br. ex Britton & P.Wilson Verbenaceae
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Advisor : LEONEIDE ÉRICA MADURO BOUILLET
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COMMITTEE MEMBERS :
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LEONEIDE ÉRICA MADURO BOUILLET
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ALAIDE BRAGA DE OLIVEIRA
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MAXWELL BARBOSA DE SANTANA
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PABLO LUÍS BAIA FIGUEIREDO
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Data: May 27, 2022
Ata de defesa assinada:
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Show Abstract
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Ethnopharmacological relevance: Lippia alba (Mill.) N. E. Brown (Verbenaceae), is a plant that is frequently utilized due to the wide chemical variation of its essential oils. The diversification of the components of essential oils is responsible for its diverse uses in traditional medicine applications. However, the wide chemical variation of chemotypes of Lippia alba is not without concern with respect to its use as a phytotherapeutic medicine since the user could be using material that is not adequate to the achieve the desired effect. Aim of the study: Evaluate if the essential oil of three specimens of Lippia alba have an inhibitory effect on the acetylcholinesterase enzyme. Methods: The volatile constituents of the essential oils obtained through hydrodistillation (L.alba1 and L.alba2) and by steam distillation (L.alba3) were determined by gas chromatography–mass spectrometry and were then submitted to acetylcholinesterase enzyme inhibition trials using the direct bioautography methods of Marston et al., (2001) and Ellman et al., (1961) with modifications. Results: The chromatographic profiles of the principle volatile constituents of the essential oil samples showed three distinct chemotypes: L.alba1 with a high content of citral (neral 23.84% and geranial 32.31%); L.alba2 with carvone (30.72%), 1,8-cineol (14.37%) and limonene (10.3%), and L.alba3 principally constituted of linalool (68.31%). All essential oil samples displayed inhibitory activity to the acetylcholinesterase enzyme, with L.alba1 showing the highest inhibition (I%: 97.9 ± 0.8), followed by L.alba2 (I%: 54.3 ± 5.6) and L.alba3-(I%: 46.9 ± 5.6). The percentage of inhibition of acetylcholinesterase shown by the isolated citral constituent (synthetic) was lower (49.3%±12.2) than for the L.alba1 chemotype, suggesting a synergism between the constituents present in the essential oils than can enhance anticholinesterase activity. Conclusions: The essential oil of L. alba is a potent inhibitor of the acetylcholinesterase enzyme, and this activity varies between moderate and high depending on the chemotype. In this way, as an aid in the tracking of phytotherapeutic raw materials, programs of plant seedling production and distribution should include botanical and chemical identification of accessions of L. alba.
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4
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CRISTIANO GONCALVES MORAIS
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MERCURIAL EXPOSURE: CLINICAL-EPIDEMIOLOGICAL WOMEN PROFILE IN THE LOWER AMAZONAS REGION
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Advisor : HELOISA DO NASCIMENTO DE MOURA MENESES
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COMMITTEE MEMBERS :
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HELOISA DO NASCIMENTO DE MOURA MENESES
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RICARDO BEZERRA DE OLIVEIRA
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LIGIA MERES VALADAO
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MARINA SMIDT CELERE MESCHEDE
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Data: Jul 7, 2022
Ata de defesa assinada:
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Show Abstract
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Mercury is a heavy metal that causes harm to human health, mercury exposure occurs through means such as burning amalgam and in the feeding of fish contaminated by mercury. Women of childbearing age, pregnant or breastfeeding are part of the risk group and must be monitored; in order above all to avoid the effects of the consumption of contaminated fish, which can generate a clinical picture of: muscle weakness, infertility, hormonal dysregulation, polycystic ovary syndrome. Furthermore, the effects of mercury exposure affect the fetus in the intrauterine period, contributing to delays and/or damage to the nervous system, congenital malformations, and miscarriages. In the Brazilian Amazon, there is an interest in investigating mercury exposure, given the anthropic mining activities in the region, as well as the presence of contaminated fish that add risks to the health of the population, mainly due to the high consumption of fish by people in the Amazon region. . Understanding that in the Lower Amazon region there is an environmentally exposed population and given the dangers that mercury exposure can cause to women, this study aims to assess the epidemiological and clinical profile of women environmentally exposed to mercury in the Lower Amazon region, Pará. This is a descriptive, cross-sectional and quantitative study, which will be carried out based on data collected from 2016 to 2019 from 327 women living in the urban and riverside area of Santarém, in the Lower Amazon region. For this, the analysis of social, demographic, food, anthropometric, clinical and laboratory variables will be carried out. As well as the analysis of the concentration of mercury in the blood, considering "exposed" women with more than 10 μg/L of mercury in their blood, following the reference values established by the World Health Organization. Descriptive statistical analysis and of these variables in the Statistical Package for Social software. It is hoped that the data acquired in this study will serve to characterize the exposure profile of women in the region, as well as indicate the dangers of mercury to future pregnancies of exposed women.
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5
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ROMULO JOSÉ DE SOUSA
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Comparative study of survival between patients with medulloblastoma operated at an oncology referral center and other hospitals.
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Advisor : MAXWELL BARBOSA DE SANTANA
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COMMITTEE MEMBERS :
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MAXWELL BARBOSA DE SANTANA
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SANDRA LAYSE FERREIRA SARRAZIN
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RICARDO BEZERRA DE OLIVEIRA
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LEOMARA ANDRADE DA SILVA
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Data: Sep 16, 2022
Ata de defesa assinada:
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Show Abstract
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Medulloblastoma (MB) is the most common brain malignancy of childhood, repre-senting approximately 15-20% of all central nervous system (CNS) tumors that affect the pediatric population. The survival of children with medulloblastoma increases significantly when the combined treatment of surgery, radiotherapy and chemother-apy is used. The main objective of this study was to evaluate survival among pa-tients diagnosed with medublastoma operated in an oncology referral center (IN-CA/RJ – Instituto Nacional de Câncer/Rio de Janeiro/RJ) in comparison with public and private hospitals that visit their patients for treatment. adjuvant (after surgery) for INCA. A retrospective cohort of both genders was described, with histopathological diagnosis of both medulloblastoma, ages between 3 and 18 years, enrolled in the Pediatric Oncology sector of the Instituto Nacional do Câncer/RJ (INCA/RJ). Most of the ethnicities described are the events that occurred between the years 2010 to 2021. Considering the data of male cases, it is possible to verify that it is male (55.4%), and mixed with 43.1% and 44, 6% respectively. In addition, 95.4% of pa-tients were selected for radiotherapy and 87.7% for chemotherapy. How many, pa-tients operated at INCA/RJ showed a lifetime about how many life groups have no meaning about this referral center and disease overall survival time, about the major-ity between the two groups studied.
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6
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CAMILA CASTILHO MORAES
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Evaluation of in vivo antimalarial activity of acetonic extract from andiroba (Carapa guianensis) industrial residue
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Advisor : WALDINEY PIRES MORAES
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COMMITTEE MEMBERS :
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ANTONIO HUMBERTO HAMAD MINERVINO
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LINCOLN LIMA CORREA
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THALIS FERREIRA DOS SANTOS
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WALDINEY PIRES MORAES
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Data: Sep 23, 2022
Ata de defesa assinada:
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Show Abstract
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Malaria is an infectious disease, neglected, endemic in more than 85 countries, responsible for more than 241 million cases, considered one of the biggest economic and social problems in the world. In Brazil, in 2020, 143,395 cases of the disease were reported. Plasmodium falciparum and Plasmodium vivax are primarily responsible for most cases of the disease. Malaria eradication is of global interest, however, the lack of a vaccine, the emergence of resistant parasites and their high endemicity make this control difficult, and justify the search for new bioactive molecules for the production of new drugs with antimalarial potential. Thus, this research evaluated the in vivo antimalarial activity of the acetone extract of Carapa guianensis, as well as its acute toxicity, survival analysis and analysis of hematological parameters. The industrial residue of Carapa guianensis was supplied by the vegetable oil extractor company AmazonOil located in the city of Ananindeua-PA. The analysis of the chemical composition of Carapa guianensis was performed using ultra-performance liquid chromatography coupled to sequential mass spectrometry (MS-MS). Assessment of in vivo toxicity in BALB/c mice was performed according to the OECD Guide. To evaluate the antimalarial activity in vivo, BALB/c mice were used, infected with approximately 106 erythrocytes parasitized by P. berghei receiving treatment on the 4th day after inoculation, treated for 7 consecutive days and submitted to blood collection to determine the hematological parameters. The chemical characterization identified limonoids as the major compounds being methyl angolensate (21.17%) and 6α-acetoxygedunin (12.38%). The acute toxic dose of acetone extract of Carapa guianensis was higher than 2000 mg/kg in BALB/c mice, being classified in category 5 of the Globally Harmonized Classification System (GHS). The doses of acetone extract (100 and 200 mg/kg/day) significantly reduced P. Berghei-induced parasitaemia, as well as obtaining an important result in the evaluation of mouse survival. In view of the above, the acetone extract of Carapa guianensis, a natural product from the Amazon, presented a safe antimalarial potential and a promising candidate for further complementary research.
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7
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DANIELE FERREIRA DA SILVA
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Reuse of Handroanthus serratifolius wood residue as a proposal for an antibacterial topical formulation on Astrocaryum murumuru vegetable butter base
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Advisor : GABRIELA BIANCHI DOS SANTOS
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COMMITTEE MEMBERS :
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SILVIA KATRINE RABELO DA SILVA
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GABRIELA BIANCHI DOS SANTOS
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MADSON RALIDE FONSECA GOMES
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EDILENE GADELHA DE OLIVEIRA
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Data: Sep 29, 2022
Ata de defesa assinada:
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Show Abstract
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There is a growing search for new natural products with antimicrobial properties to combat multi-resistant bacteria such as Staphylococcus aureus, responsible for several diseases, including dermatological infections. Some plants used in traditional medicine are candidates in the prospection of antibiotic bioactives, with emphasis on Handroanthus serratifolius (yellow ipê) that have diverse biological properties, due to their chemical composition with naphthoquinones and lapachol. This study aimed to obtain and characterize plant extracts from the reuse of wood residue from H. serratifolius to develop a semi-solid formulation with antibacterial action. For this, It was used the sawdust of H. serratifolius that presented moisture content and total ash within acceptable limits to ensure sample quality and moderately coarse dust classification. Three extracts were obtained, aqueous from the decoction of the sample, hydroalcoholic from maceration in 70% alcohol and ethanolic from maceration in absolute ethyl alcohol. They were lyophilized to generate crude dry extracts, whose phytochemical characterization showed quinones, anthraquinones and steroids. Only in aqueous extract revealed tannins. It was used thin layer chromatography in the chemical composition of all extracts to verify the presence of lapachol. Subsequently, these were submitted to the antimicrobial test to detect the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against pathogenic skin bacteria: S. aureus, S. aureus MRSA, S. pyogenes, E. faecalis, E. coli and P. aeruginosa, where gram-positive bacteria were showed sensitive. The aqueous extract stood out with better bactericidal performance in the largest number of strains tested, with S.pyogenes being more sensitive with MIC of 0,156 mg/mL and MBC of 0,625 mg/mL. Thus, 1% of the aqueous dry extract was incorporated into the semi-solid formulation based on Astrocaryum murumuru butter, with a pH of 5,8, compatible for use on skin and mucous membranes. The polarized light microscopy of the formulation revealed the presence of liquid crystals with a hexagonal phase, which could allow the sustained release of the actives for a longer period of time. The formulation was shown to be stable for 90 days, regardless of storage temperature, and maintained antibacterial activity against bacteria sensitive to the aqueous extract. Comparisons between a formulation with 1% of extract and a formulation with 0.5% of lapachol, under the same conditions, it was shown that both exhibit satisfactory antibacterial action against S. aureus, whose extract is as efficient as the isolated substance (p> 0.05). In silico prediction to skinsensitization, the components identified in the formulation, the lapachol presented a skin sensitizer alert to susceptible individuals, by the Bayesian method. However, there were no signs of toxic skin risk in the Toxtree database, thus allowing for the assurance of topical use. Therefore, this study contributed to highlight the alternative proposal of an antibacterial herbal formulation for skin infections, emphasizing the quality of waste discarded by the wood sector as a potential source of extraction of bioactive compounds.
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8
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PATRÍCIA GABRIELLY DA SILVA PIRES
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Anti-edematogenica and antiniceptive activity of Pectis elongata Kunth essential oil
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Advisor : RICARDO BEZERRA DE OLIVEIRA
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COMMITTEE MEMBERS :
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ELENN SUZANY PEREIRA ARANHA
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LEOMARA ANDRADE DA SILVA
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MAXWELL BARBOSA DE SANTANA
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RICARDO BEZERRA DE OLIVEIRA
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Data: Oct 19, 2022
Ata de defesa assinada:
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Show Abstract
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The present work aims to investigate the anti-inflammatory and analgesic properties of the essential oil of Pectis elongata Kunth; an aromatic herb belonging to the Asteraceae family, widely used by traditional communities to treat pain and inflammation. It has in its composition two major components, citral isomers (neral and geranial), monoterpenes with anti-inflammatory and analgesic action already described in the literature. The main hypothesis investigated in this work is that the essential oil obtained from P. elongata can serve as an alternative to standard therapy for the treatment of pain and inflammation, since these classes of conventional drugs can have many adverse reactions leading to non-adherence to the treatment by the patients. The identification of the neral and geranial components, present in other plant species, as responsible for their analgesic and anti-inflammatory properties established in the literature, is a strong evidence that Pectis elongata, a native and abundant species in the Amazon, could serve as a solution to problem presented. This hypothesis will be investigated through in vivo tests of paw edema induced by λ-carrageenan, to identify anti-inflammatory action and formalin test, where antinociceptive action (pain) is verified. Rats (n=30) of the Wistar lineage will be used for the paw edema test and mice (n=30) of the Swiss lineage will be used for the formalin test, which will be divided into 6 groups with 5 animals each. The results will be statistically analyzed, in both tests, by analysis of variance ANOVA followed by Tukey test, with confidence intervals of 5%. Values of p ≤ 0.05 will be considered statistically significant.
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9
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GESSICA ALEANE MORAES ESQUERDO
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EVALUATION OF IN VITRO ENZYMATIC INHIBITION AND IN VIVO ANTIDIABETIC ACTIVITY OF ESSENTIAL OIL FROM Cyperus articulatus L.
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Advisor : TANIA MARA PIRES MORAES
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COMMITTEE MEMBERS :
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MARINA SMIDT CELERE MESCHEDE
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MAXWELL BARBOSA DE SANTANA
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TANIA MARA PIRES MORAES
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TIAGO SANTOS SILVEIRA
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Data: Oct 27, 2022
Ata de defesa assinada:
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Show Abstract
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Diabetes mellitus is a metabolic disease resulting from defects in insulin secretion or action, being a chronic condition and a serious public health problem that can cause serious complications that can lead to death. It is important to emphasize the high costs related to its control with treatment and the numerous side effects caused by medications and the popular use of medicinal plants, highlighting the need to develop new bioactive molecules for the production of new drugs with antidiabetic potential to treat this disease. In this context, the present work studied the essential oil obtained from the rhizomes of Cyperus articulates L. (OECA) to investigate its chemical composition, acute oral toxicity, in vitro enzyme inhibition and in vivo antidiabetic activities, respectively, and to support its possible use as a therapeutic alternative in the treatment of diabetes. The analysis of the chemical composition of the OECA collected in the experimental farm of UFOPA, in the region of Tabocal in the municipality of Santarém, Pará, Brazil, was carried out using an Agilent HP-6890 gas chromatograph, the evaluation of ACUTE oral toxicity in vivo in mice Swiss according to the OECD Guide, after the enzymatic inhibition in vitro, using the enzymes α-glucosidase and lipase, and their readings were made in a spectrophotometer, in vivo using mice of the Swiss strain that were induced to experimental diabetes with the diabetogenic drug alloxan and treated with OECA by gavage during 14 days of treatment, blood glucose and physiological parameters were measured during the 3rd, 7th and 14th day of treatment and blood samples were collected to determine the biochemical parameters. Chemical characterization by GC-MS allowed the identification of 24 compounds with mustacone as the major compound. The acute toxic dose of OECA is greater than 2000 mg/kg in Swiss mice, classifying it in category 5 of the Globally Harmonized Classification System (GHS). In vitro, OECA has a low potential for enzymatic inhibition with the mean (IC50 =6.4%) against the α-glucosidase enzyme and the mean (IC50 =31.1%) against the lipase enzyme. In vivo, it significantly reduced (p< 0.001) blood glucose and, consequently, improved biochemical parameters at doses of 100 and 200 and 400 mg/kg/day. Therefore, OECA is a natural product extracted from the Amazon that presented safe, promising antidiabetic potential and a candidate for the development of new drugs, requiring further studies.
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HERMAN ASCENÇÃO SILVA NUNES
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NANOEMULSION CONTAINING ESSENTIAL OIL FROM Pectis elongata Kunth: ANTIMICROBIAL, ANTI-INFLAMMATORY AND STIMULANT POTENTIAL SKIN WOUND HEALING PROCESS
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Advisor : SANDRA LAYSE FERREIRA SARRAZIN
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COMMITTEE MEMBERS :
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LEOMARA ANDRADE DA SILVA
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RICARDO BEZERRA DE OLIVEIRA
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SANDRA LAYSE FERREIRA SARRAZIN
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TIAGO SANTOS SILVEIRA
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Data: Nov 4, 2022
Ata de defesa assinada:
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Show Abstract
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Pectis elongata Kunth, Asteracea, is an herbaceous plant, known in Northern Brazil as "wild cuminho", "lemon rosemary", "cuminho" or "wild rosemary". It is a species rich in essential oil, whose major constituent is citral, a component with recognized antifungal, anti-inflammatory, analgesic, sedative, antibacterial properties, among others. In this project, our proposal is to evaluate the efficiency of the nanoemulsification process of P. elongata essential oil on the retention of its main volatile constituents and antimicrobial, anti-inflammatory and stimulating properties of the healing process of skin wounds. For this, in the first step, already carried out, the essential oil of P. elongata was found by hydrodistillation and the nanoemulsification process carried out by the low-energy method. The essential oil yield was determined in 1% w/v. The chemical composition of the free and nanoemulsified oil was provided by gas chromatography coupled with mass spectrometry, which pointed out citral as the major constituent for both as analyzed: free (92,5%) and nanoemulsified oil (94,8%, 96,8% e 82%, respectively). In the next step, we will carry out the analysis of the chemical composition of the nanoemulsion at times 30 and 60 days after preparation. The antimicrobial potential of the essential oil and nanoemulsified will be evaluated by agar disk-diffusion and broth microdilution methods, the anti-inflammatory potential will be evaluated by the carrageenan-induced paw edema test and the stimulating potential of the healing process evaluated on skin wounds induced by surgical incision. We hope that the P. elongata essential oil nanoemulsification process will be effective both in maintaining its chemical properties and in preventing the growth of microorganisms, modulating the inflammatory response and promoting the healing process of skin wounds.
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