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Disertaciones |
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1
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MILENA DE SOUSA VASCONCELOS
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Evaluation of the in vivo Pharmacological Activity of Essential Oil of Piper marginatum (PIPERACEA)
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Líder : MAXWELL BARBOSA DE SANTANA
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MIEMBROS DE LA BANCA :
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MAXWELL BARBOSA DE SANTANA
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SÂMIA RUBIELLE SILVA DE CASTRO
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TANIA MARA PIRES MORAES
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Data: 06-feb-2019
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Resumen Espectáculo
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Piper margintum, in the Piperacea family is a native and endemic plant in Brazil. It is an important plant used medicinally in South and Central America. The most common use of Piper marginatum is related to gastrointestinal diseases. The aim of this study was to evaluate the possible gastroprotective and antiedematogenic effects of Piper marginatum Essential Oil. For antiedematogenic effect was used the ear edema induced by Phenol. For the gastroprotective effect was used the gastric injury induced by acified ethanol and Indomethacin. It was observed after 1 hour of edema induction, the OEPM inhibited edema both at the dose of 1000 mg / kg (55%) and 500 mg / kg (42%) when compared to the Phenol group. In the acidified acid-induced gastric injury test, the OEPM showed a lower percentage of ulcerated area at the doses of 1000mg / kg (7.90%) and 500 mg / kg (8.41%), when compared to the control group (17.92%). In the indomethacin-induced gastric test, a lower percentage of ulcerated area was observed in the groups which received the OEPM; however, there was no statistically significant difference at 1000 (9.02%), 500 (11.61%) and 250 mg / kg (15.08%), when compared to the control group (16.92%). The results presented in this study showed the OEPM has active compounds with gastroprotective and antiedematogenic effects.
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2
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ARIMAR CHAGAS DE ALMEIDA
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ANTIMALARIAL ACTIVITY OF Kalanchoe pinnata ON Plasmodium berghi IN EXPERIMENTAL MODEL IN VIVO
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Líder : WALDINEY PIRES MORAES
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MIEMBROS DE LA BANCA :
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WALDINEY PIRES MORAES
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BRUNO ALEXANDRE DA SILVA
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JOSAFA GONÇALVES BARRETO
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Data: 20-feb-2019
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Resumen Espectáculo
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Malaria is an infectious disease caused by a microscopic parasite, which infects about 200 million people a year. It presents as a cyclic fever followed by chills, intense sweating, nausea, vomiting, pallor and physical weakness, which intensify according to the level of parasitemia and the species of Plasmodium that infect individuals. Despite being a worldwide problem, malaria does not present a homogeneous geographic distribution, being its higher incidence in tropical and subtropical regions such as the Legal Amazon in Brazil. Currently, a range of drugs used to treat malaria has become inefficient because some strains of Plasmodium vivax and Plasmodium falciparum already have multiple resistance to these drugs. In this context, it is necessary to seek new therapeutic alternatives for the treatment of malaria, such as plants. Among the thousands of plant species, Kalanchoe pinnata is a plant that presents indicative of hypotensive, anti-inflammatory and antirheumatic effect. Thus, the possibility of researches with the extract of Kalanchoe pinnata, widely used in the North of Brazil for the treatment of inflammations, wounds, cutaneous ulcers, abscesses, analgesia and also antiparasitic activity against Leishmania amazonensis. In view of this, the present study aims to evaluate the antimalarial activity of the hydroalcoholic extract of Kalanchoe pinnata in an in vivo model of malaria using BALB/c mice infected with Plasmodium berghei. To this end, the hydroalcoholic extract of Kalanchoe pinnata was evaluated for its toxicity and effects (suppression of parasite density, animals survival time, plasma glucose levels, liver and renal function and haematological parameters) in the treatment of BALB/c mice infected with Plasmodium berghei. Thus, K. pinnata extract was safe for treatment with the studied doses, showing similar effect of the standard drug artemisinin, such as increased survival time, parasitic suppression, control of glycemic levels and renal and hepatological functions, besides haematological parameters. Thus, it has been shown that this extract presents itself as an effective alternative for the treatment of malaria.
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3
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ANDREW MAIROM NOGUEIRA PEREIRA
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ANTI-INFLAMMATORY AND ANTICIOCYPTIVE ACTIVITY OF THE ESSENTIAL OIL OF Cyperus articulatus var. nodosus L
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Líder : WALDINEY PIRES MORAES
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MIEMBROS DE LA BANCA :
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WALDINEY PIRES MORAES
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TANIA MARA PIRES MORAES
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JOSAFA GONÇALVES BARRETO
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Data: 20-feb-2019
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Resumen Espectáculo
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The Cyperus articulatus var. nodosus L. (Priprioca) is a plant found in the Amazon region due to the biodiversity of plant species in this place and has become important source in the discovery of new drugs. Phytochemical studies carried out on essential oil have been identified from terpene compounds. Based on the presence of the compounds present in Cyperus articulatus var. nodosus L. grows the hypothesis of having anti-inflammatory and antinociceptive activity. Phytochemical study on essential oil identified the presence of terpene compounds. Considering the chemical compounds present in the essential oil of Cyperus articulatus var. nodosus (OECA) and ethnopharmacological application, the objective of the research was to evaluate the anti-inflammatory and antinociceptive activity of the essential oil of Cyperus articulatus var. nodosus in experimental models of inflammation and nociception in rats and mice. The botanical material was provided by the Research and Development Laboratory of bioactive natural products of UFOPA, which was collected in Tabocal region in the municipality of Santarém-PA, identified by Dr. Antônio Elielson Sousa da Rocha. The chemical characterization of the sample was carried out at the multidisciplinary center for chemical, biological and agricultural research of UNICAMP-SP. The assessment of acute oral toxicity was assessed according to OECD protocol 423/2001. The antinociceptive activity has followed the protocols of the Hot Plate, Abdominal Contortion and Formalin tests. The anti-inflammatory activity was evaluated with the Airbag and Evanz Blue tests. The chemical composition analysis indicated the presence of monoterpenes, sesquiterpenes and sesquiterpene ketones as major constituents of OECA. The safe dose is less than 2,000 mg / kg administered orally. It presents antinociceptive activity increasing the pain threshold induced by chemical substances (acetic acid and formalin) and thermal stimulation. Anti-inflammatory activity is observed, decreasing the exudate volume at the inflamed site as a consequence of the decrease in vascular permeability observed in the air bag and Evanz Blue test. The anti-inflammatory effect was also observed in the reduction of leukocyte migration in the air bag test.
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4
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ANTÔNIO QUARESMA DA SILVA JÚNIOR
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ESSENTIAL OIL OF Lippia alba (MILL.) N. E. BROWN: SEASONAL VARIATION, ANTIMICROBIAL ACTIVITIES, ANTIOXIDANT AND ITS APPLICATION IN A
FORMULATION FOR INHIBITION OF PATHOGENIC MICROORGANISMS.
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Líder : ROSA HELENA VERAS MOURAO
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MIEMBROS DE LA BANCA :
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ROSA HELENA VERAS MOURAO
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KARIANE MENDES NUNES
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ALCICLEY DA SILVA ABREU
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Data: 18-mar-2019
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Resumen Espectáculo
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Many studies have reported a large variation of the chemical constituents of the essential oil (EO) of Lippia alba (Mill.) N.E. Brown, with seasonality, genetic and environmental factors being the main responsible for these differences. Thus, the objective of this work was to evaluate the influence of seasonality and time of harvest on the yield, chemical composition and antimicrobial and antioxidant activities of L. alba essential oil grown in Santarém, Pará, Brazil, in addition to developing a topical use formulation containing EO with antimicrobial activity. Leaves of L. alba were collected during the rainy season and the dry season at 8 a.m, 12 p.m and 5 p.m in 2014 and during the period from January 2016 to January 2017. Climatic parameters were monitored throughout the harvest period. EO was obtained by hydrodistillation and its chemical components were analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The formulation of topical use was prepared with the incorporation of 10% of the EO to an anionic base of the type Lanette® and submitted to evaluation of its stability. The antibacterial activity against S. epidermidis (CCCD S010) of the formulation and EO was performed by disc diffusion agar and broth microdilution methods. For the antioxidant activity of EO, was used the DPPH radical sequestration method. The main chemical constituents of EO (above 2%) were submitted to multivariate statistical analysis (PCA, Principal Component Analysis, HCA, Hierarchical Cluster Analysis). The average yield of EO ranged from 2.2 to 4.3% in 2014, with the highest percentage for the rainy season (in March), whereas in 2016 it varied from 1.6 to 4.5%, with significant variation due to seasonality. The HCA and PCA analyzes confirmed the influence of seasonality on the chemical composition of L. alba EO. Rainfall and solar radiation may be the main factors that contributed to the variation of some chemical components of EO during the months of the year. However, the major chemical compound of the species is always citral, regardless of the seasonal period and time of harvest, which characterizes its chemotype. The formulated product containing the oil remained about 45% citral and showed stability at temperatures up to 25 °C. In the centrifugation test no changes were observed regarding homogeneity, indicating a good compatibility between the base and the EO. The inhibition halo against S. epidermidis was 16 ± 1.73 mm (at 5 °C) and 7.16 ± 0.28 mm (at 25 °C) for the formulation and > 45 mm for pure EO in both periods. It was observed higher EO effectiveness in the rainy season with MIC of 1.25 μl/ml and 2.5 μl/ml in the dry season. In the antioxidant activity, the samples presented low antioxidant capacity, with percentages of DPPH inhibition varying from 0.97 ± 0.16% to 2.87 ± 0.68%, without significant influence of seasonality. According to the results, the harvest period influenced the yield, chemical composition and antimicrobial activity of L. alba EO. The formulation containing EO presented a good stability and has considerable antimicrobial action against S. epidermidis, which stimulates the continuity of the studies.
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5
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ANTÔNIO SAMUEL GARCIA DA SILVA
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MORPHOMETRIC VARIATION OF THE HEAD OF Bothrops atrox: COMPARATIVE ASPECTS BETWEEN SPECIES OF DIFFERENT HABITATS OF THE WEST REGION OF PARÁ
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Líder : ROSA HELENA VERAS MOURAO
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MIEMBROS DE LA BANCA :
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ROSA HELENA VERAS MOURAO
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ALFREDO PEDROSO DOS SANTOS JUNIOR
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LEONEIDE ÉRICA MADURO BOUILLET
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Data: 22-mar-2019
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Resumen Espectáculo
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Bothrops atrox (Viperidae) is a nocturnal venomous snake in its diet and habitat use. This species is widely distributed throughout the Amazon, inhabiting diverse habitat types such as floodplain areas, primary and secondary forests, savanas, and degraded areas. Studies have shown variation in the composition of venom in flooded forest specimens of floodplain, which is possibly an adaptation to types of prey in these areas. The availability and composition of prey are also expected to cause morphometric variations, especially determined by characters that are measured in the head. The objective of this study was to analyze the morphometric characteristics of Bothrops atrox venom head and glands among specimens of the different habitats of the western region of Pará. Specimens of várzea area collected in the Igarapé do Costa community were used for analysis Urucurituba and Santa Maria do Tapará in Santarém, Pará, and specimens of non-flooded areas from the Floresta Nacional do Tapajós in Belterra (Florestal), Alter do Chão (Savana), Santarém and Jatuarana (Degradada) communities in Oriximiná, Pará. We analyzed 107 adult specimens of Bothrops atrox, 54 males and 53 females, comprising 53 specimens of várzea and 54 of non - flooded forests. Data collection was carried out by means of photos of the dorsal region and right side of the head of all the specimens, and analyzed from the ImageJ 1.46 software. In addition, venom glands were weighed on precision scales individually. The variables (except gland weight) were represented by the residuals of linear regression models, used to reduce the effects of body size (face-cloacal length) of snakes. A non-parametric Kruskal-Wallis analysis, complemented with Dunn's post hoc test, was used to analyze the morphometric differences presented by B. atrox specimens among habitats, while a Principal Component Analysis (PCA) was performed for decrease the dimensionality of the data, producing a smaller number of variables to represent the variations between the groups. The morphometric analyzes in the head of B. atrox of this study showed significant differences in seven of the 14 variables analyzed. Differences in the width and area of the head and venom glands were shown, as well as differences in weight of venom glands. All variations were between wetland and non-wetland specimens. These findings are possibly related to the different types of prey available in these habitats, despite the scarcity of studies on the diet of this species in lowland areas. Therefore, in this study, flooded forests pitviper presented smaller head size, as well as smaller and lighter venom glands in relation to the specimens of the other sampled habitats (Forest, Savanna and Degraded), demonstrating an intraspecific variation in head morphology among specimens of B. atrox of different habitats of the western region of Pará.
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6
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HEVELINE CAMPOS PEREIRA
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GENOTOXIC AND HISTOLOGICAL EVALUATION IN FISH (Hyphessobrycon heterorhabdus) OF IGARAPÉ CARARAZINHO EXPOSED TO CHORUM FROM LANDFILL DUMP SANTARÉM - PA.
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Líder : LUIS REGINALDO RIBEIRO RODRIGUES
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MIEMBROS DE LA BANCA :
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LUIS REGINALDO RIBEIRO RODRIGUES
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MAXWELL BARBOSA DE SANTANA
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MARCOS PRADO LIMA
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Data: 25-mar-2019
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Resumen Espectáculo
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The enormous waste production is one of the major environmental issues in the present. The waste inappropriate discard and management give serious risks to the human and ecosystem health. The leachate, liquid produced by garbage decomposition, can contain many molecules potentially damaging to genetic material, such as heavy metals and aromatic polycyclic hydrocarbon (APH). The present study goals to evaluate the genotoxic and histopathological effects of the leachate from Santarém Landfill Dump (Lixão do Perema) in fishes Hyphessobrycon heterorhabdus. Micronucleus, erythrocyte nuclear abnormalities techniques and comet assay were conducted to genotoxicity evaluation, while tissue injuries were assessed with gills and liver histological preparations. The fishes were exposed to leachate (10, 5 and 2%), to natural stream Cararazinho’s water and to 20ppm benzene. The genotoxicity biomarkers do not evidenced significant variation between exposed and control group (stream natural water). Gills and liver showed diverse types of tissue lesions. Therefore, under the light of these results we conclude that Cararazinho’s stream water, because receives leachate from the Santarém Landfill Dump, plays to the occurrence of genotoxic and tissue damages in fishes H. heterorhabdus.
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7
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LUAN AÉRCIO MELO MACIEL
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CYTOGENOMIC STUDY IN Hypostomus soniae Carvalho & Weber, 2005 (SILURIFORMES - LORICARIIDAE) OF TAPAJÓS RIVER, PARÁ, BRAZIL
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Líder : LUIS REGINALDO RIBEIRO RODRIGUES
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MIEMBROS DE LA BANCA :
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CARLOS IVAN AGUILAR VILDOSO
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FRANK RAYNNER VASCONCELOS RIBEIRO
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LUIS REGINALDO RIBEIRO RODRIGUES
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Data: 29-mar-2019
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Resumen Espectáculo
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The genus Hypostomus (Loricariidae-Hypostominae) encompasses around 200 valid species, being characterized by presenting high karyotypic diversity, with diploid numbers from 2n-64 in H. aff. Cochliodon to 2n=84 in Hypostomus sp. The present study aims to investigate the karyoevolution of H. soniae from Tapajós River. Were analyzed ten specimens (6F, 3M, 1 undetermined). Were used techniques of Giemsa staining, C-banding, Ag-NOR, Cromomycin A3 (CMA3) and fluorescent in situ hydribridization (FISH) with rDNA 5S and 18S, telomeric, histone H1 and H3, snRNA U2 probes. The results revealed karyotype with 2n=64, NF=112 e FC=12m+22sm+14st+16a. The C-banding evidenced heteromorphism of heterochromatic blocks in the pairs 25 and 26, that may related with development of a nascent XX/XY sex chromosome system. NORs were detected in the telomeric regions of an acrocentric, except to the specimen PMT-36 that showed two labeled pairs, in agreement with CMA3 and rDNA 18S evidences. Three pairs showed marks to rDNA 5S probes and the telomeric probes labeled the extremity of all the chromosomes. We mapped for the first time H1 and H3 histones and snRNA U2 in the karyotype of H. soniae, and then H1 and H3 revealed clusters in two chromosomal pairs, pointed to conservative status of these sequences; snRNA U2 probes were detected in the distal portions in the most of the chromosomes. The results confirm the conservatism of H. soniae karyotypic macrostructure between Tapajós and Teles Pires Rivers populations and reinforces the hypothesis of occurrence of sex chromosome system and intraspecific polymorphism.
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8
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THAÍS SENA DE TROLLY
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EVALUATION OF GENOTOXICITY IN FISH OF TWO PORT AREAS OF THE TAPAJÓS RIVER, IN THE WEST OF PARÁ
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Líder : LUIS REGINALDO RIBEIRO RODRIGUES
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MIEMBROS DE LA BANCA :
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LUIS REGINALDO RIBEIRO RODRIGUES
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RUY BESSA LOPES
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MARCOS PRADO LIMA
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Data: 25-abr-2019
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Resumen Espectáculo
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The port zone are essentials to the socioeconomic development of the Amazon region, however, it can contributes to disturbing aquatic ecosystems by the intensive shipment drive and occasional discharge of pollutants. Among the chemical species that are harmful to the environment and associated with the port activities stand out the heavy metals and petroleum derivatives. Such xenobiotic molecules induces genotoxic damages to aquatic biota. The present study aims to investigate the possible genotoxic effects in fishes Pimelodus blochii and Geophagus proximus in situ exposed to aquatic pollutants in the port zones of Santarém and Itaituba cities, situated in the Tapajós Rivers, western Pará State. Therefore were collected 60 fishes, G. proximus (n=39) and P. blochii (n=21) from the both localities. The micronucleus test and counts of erithrocyte nuclear abnormalities were adopted for detection of genetic material losses and nuclear membrane lesions, while the comet assays was applied to evaluate DNA damages. P. blochii from the Itaituba port zone showed larger index of damage to DNA, while G. proximus from Santarém showed greater frequencies of micronucleus and nuclear abnormalities. A comparison between both species revealed that G. proximus is more susceptible to the xenobiotics found in the study area. The last increasing in the port sector in the Tapajós system may be associated with the high level of genotoxic damages observed in fishes from the Santarém area. On the other hand, the prolonged exposition to mining residuals and urban garbage could explain the high level of DNA damage observed in P. blochii from the Itaituba area. Biomonitoring and studies of water quality are recommended in view of the projections pointing to the growth of port sector in the western Pará State.
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9
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THAÍS SENA DE TROLLY
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EVALUATION OF GENOTOXICITY IN FISH OF TWO PORT AREAS OF THE TAPAJÓS RIVER, IN THE WEST OF PARÁ
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Líder : LUIS REGINALDO RIBEIRO RODRIGUES
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MIEMBROS DE LA BANCA :
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LUIS REGINALDO RIBEIRO RODRIGUES
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MARCOS PRADO LIMA
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RUY BESSA LOPES
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Data: 26-abr-2019
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Resumen Espectáculo
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The port zone are essentials to the socioeconomic development of the Amazon region, however, it can contributes to disturbing aquatic ecosystems by the intensive shipment drive and occasional discharge of pollutants. Among the chemical species that are harmful to the environment and associated with the port activities stand out the heavy metals and petroleum derivatives. Such xenobiotic molecules induces genotoxic damages to aquatic biota. The present study aims to investigate the possible genotoxic effects in fishes Pimelodus blochii and Geophagus proximus in situ exposed to aquatic pollutants in the port zones of Santarém and Itaituba cities, situated in the Tapajós Rivers, western Pará State. Therefore were collected 60 fishes, G. proximus (n=39) and P. blochii (n=21) from the both localities. The micronucleus test and counts of erithrocyte nuclear abnormalities were adopted for detection of genetic material losses and nuclear membrane lesions, while the comet assays was applied to evaluate DNA damages. P. blochii from the Itaituba port zone showed larger index of damage to DNA, while G. proximus from Santarém showed greater frequencies of micronucleus and nuclear abnormalities. A comparison between both species revealed that G. proximus is more susceptible to the xenobiotics found in the study area. The last increasing in the port sector in the Tapajós system may be associated with the high level of genotoxic damages observed in fishes from the Santarém area. On the other hand, the prolonged exposition to mining residuals and urban garbage could explain the high level of DNA damage observed in P. blochii from the Itaituba area. Biomonitoring and studies of water quality are recommended in view of the projections pointing to the growth of port sector in the western Pará State.
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10
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ÉDEN BRUNO SOUSA DA SILVA
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Evaluation Of Anti-Inflammatory, Antiproliferative And Production Of Reactive Oxygen Species Of The Ethanolic Extract Of Cyperus Articulatus L. (Cyperaceae)
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Líder : MAXWELL BARBOSA DE SANTANA
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MIEMBROS DE LA BANCA :
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DEBORA KONO TAKETA MOREIRA
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FLAVIA GARCEZ DA SILVA
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MAXWELL BARBOSA DE SANTANA
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Data: 27-may-2019
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Resumen Espectáculo
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Priprioca (Cyperus articulatus L.) is a medicinal plant traditionally used against inflammation. Studies on the essential oil of this species have led to the identification of many terpene compounds. Little is known about the use of the solid residues generated after the extraction of the essential oil of this species. This work aimed to investigate the chemical composition and to evaluate the anti-inflammatory, antioxidant and antiproliferative activity in vitro of the ethanolic extract of solid residues generated from the extraction of the essential oil of Cyperus articulatus L., in peritoneal macrophages of mice and cell lines tumor cells. The following methodology was used to achieve these objectives: chromatographic analysis, cell viability, nitric oxide production, prostaglandins type 2, interleukin 1 beta, tumor necrosis factor alpha and reactive oxygen species, arginase activity, antiproliferative activity. Results: The chemical composition analysis indicated the presence of monoterpenes, sesquiterpenes and especially sesquiterpene ketones as major constituents. The results showed that the treatment with ethanolic extract of Cyperus articulatus L. decreases the production of reactive species of oxygen, nitric oxide, prostaglandins type 2, interleukin 1 beta, tumor necrosis factor alpha, activity of the arginase enzyme and proliferation of cancer cells. The ethanolic extract of Cyperus articulatus L. also did not demonstrate cytotoxicity at concentrations of 12.5, 25 and 50 μg/mL. The results indicated that the extract of Cyperus articulatus L. exerts antioxidant, antiproliferative and anti-inflammatory activity in experimental models in vitro.
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11
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JOSIANE ELIZABETH ALMEIDA E SILVA
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CHEMICAL CHARACTERIZATION, MICROBIOLOGICAL EVALUATION AND DEVELOPMENT OF A FORMULATION WITH Copaifera reticulata Ducke OIL
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Líder : ELAINE CRISTINA PACHECO DE OLIVEIRA
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MIEMBROS DE LA BANCA :
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ELAINE CRISTINA PACHECO DE OLIVEIRA
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KARIANE MENDES NUNES
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SILVIA KATRINE RABELO DA SILVA
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Data: 28-jun-2019
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Resumen Espectáculo
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The biological properties of medicinal plants are attributed to their chemical potential which performs some activities already described in the literature. Among them is the antimicrobial activity, which has been studied due to the increase of bacterial resistance to multiple antimicrobial drugs. In view of the demand for new therapeutic alternatives, medicinal plants represent an important source for obtaining these medicines. Copaiba oleoresin has several proven medicinal properties, among them antimicrobial activity. Therefore, the aim of this work is to evaluate the chemical composition, cytotoxicity and microbiological activity against strains Staphylococcus aureus; S. aureus resistant to methicillin and S. simulans in different seasonal periods (dry and rainy), as well as to develop a semi-solid phytotherapeutic formulation of the oleoresin (in natura) of Copaifera reticulata Ducke. This work carried out trials that evaluated the chemical composition of oleoresin by GC-MS, cytotoxic activity in vitro using VERO cells, the microbiological activity by well diffusion and microdilution in plates and developed a semi-solid formulation using an emulsified system. In relation to the results found, it was possible to identify (2S and C, 3S and C), in the chemical analysis that β-caryophyllene was the major component in all the samples used, samples 2C, 2S, 3C and 3S presented cytotoxicity only at the concentration of 200 μg / mL, and the sample 1 C and 1 S did not show cytotoxicity at any of the concentrations used, in all the bacteria used found inhibition halos indicating that there was action of the oleoresin. The determination of the Minimum Inhibitory Concentration (MIC) showed that the oleoresin collected during the rainy season showed strong inhibition, whereas in the dry period it showed a weak inhibition. The formulation incorporated with the oleoresin was stable, gel-like, light yellow in color and woody odor, with appearance of anisotropy and birefringence, with texture in the form of striations, characteristic of hexagonal phase that has been used in some studies to sustained release of Drugs. The results obtained in the present work reinforce even more the importance of copaíba oleoresin from the Amazon region as a natural herbal medicine with antibacterial potential, being thus a promising source for the elaboration of new antimicrobial drugs.
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12
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WANDERSON FERNANDES SILVA
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Effects of the association of low potency and high frequency laser therapy on tissue damage repair laser therapy
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Líder : ADRIANA CAROPREZO MORINI
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MIEMBROS DE LA BANCA :
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ADRIANA CAROPREZO MORINI
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JUAREZ DE SOUZA
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MAXWELL BARBOSA DE SANTANA
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Data: 02-jul-2019
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Resumen Espectáculo
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Introduction: Ulcers represent an important intercurrence of human health judged by the social and economic aspect due to the long time and difficulty in cicatrization. Objective: To evaluate the effect of the 660nm low power laser associated with high frequency therapy on the healing process and tissue repair in cutaneous ulcers. Materials and methods: The research was carried out in the laboratories of the Federal University of the West of Pará, State University of Pará and Esperança Institute of Higher Education in Santarém. The research period was between November and November 2018. Twenty Wistar rats were divided into 4 groups, each consisting of 5 animals each. Group GC (Control Group - received the lesion, however, without any treatment). Group GL (Laser therapy group - received the lesion and was treated with Laser therapy). Group GA (High frequency group - received the lesion and was treated with High frequency). Group GL + Al (Group Lasertherapy and high frequency - received the lesion and was treated with laser therapy and associated high frequency). After the occurrence of the lesion, the animals started pre- determined daily therapy sessions for each group, established randomly, for ten consecutive days. Euthanasia of the animals occurred on the 11th day after the initial lesion. Results: The GC group presented 0.2102 ± 0.011 cm of area on the 1st day with reduction to 0.0130 ± 0.008 cm on the 11th day. The GL group presented 0.2126 ± 0.003 cm of area on the 1st day with reduction to 0.0022 ± 0.002 cm on the 11th day. The LAG group presented 0.2092 ± 0.009 cm of area in the 1st day with reduction to 0.0212 ± 0.006 cm on the 11th day. The GL + AL group presented 0.2118 ± 0.003 cm of area on the 1st day with reduction to 0.0086 ± 0.002 cm on the 11th day. It was possible to observe significantly (p <0.05) lower lesion area in GL compared to CG group. It was also possible to observe significant reduction (p <0.01) of GL compared to LAG and LAGGL compared to LAG (p <0.01). There was no significant difference between the types of collagens in each group analyzed (p> 0.05). Conclusion: The low power laser is able to efficiently modulate the process of repairing skin wounds based on macroscopic and microscopic aspects. The high frequency generator is not able to efficiently modulate the skin wounds repair process based on macroscopic aspects, however it improves the repair process on skin wounds based on microscopic aspects. The low power laser associated with the generator of high frequency present good results in the process of tissue repair.
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13
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VICTOR HUGO RABELO DE AQUINO
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DEVELOPMENT OF BUTTER-BASED PHYTOTERAPIC VAGINAL GEL OF Astrocaryum murumuru Mart.CONTAINING OLEORRESIN OF Copaifera reticulata Ducke FOR THE TREATMENT OF VAGINOSIS
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Líder : KARIANE MENDES NUNES
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MIEMBROS DE LA BANCA :
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EDILENE GADELHA DE OLIVEIRA
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GABRIELA BIANCHI DOS SANTOS
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KARIANE MENDES NUNES
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SILVIA KATRINE RABELO DA SILVA
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Data: 12-jul-2019
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Resumen Espectáculo
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The copaiba is a plant widely used in folk medicine, studies have evidenced several biological activities, mainly antimicrobial. The use of natural products becomes an effective and economical alternative to avoid the inappropriate use of antibiotics. The search for new drug delivery systems has been of great relevance in order to make the therapy more efficient, among these release systems the liquid crystals stand out due they show some technological advantages, promoting greater stabilities against extrinsic factors and allows sustained release of drugs and / or actives. The objective of this study was to develop an phytotherapic antimicrobial gel gel of Astrocaryum murumuru Mart. butter containing Copaifera reticulata Ducke. oil-resin. The Copaifera reticulata oleoresin was collected in the Tapajós National Forest, and its chemical composition was analyzed by Gas chromatography mass spectrometry (GC-MS). The oleoresin antimicrobial activity was performed by the disc diffusion technique, using standard ATCC strains of bacteria related to vaginosis and evaluated the minimum inhibitory concentration (MIC). Five groups of emulsified systems were obtained from the fixed amount of the Procetyl surfactant with different concentrations of water and murumuru butter, with different concentrations of incorporated oleoresin. After 24 hours of preparation of the formulations, polarized light microscopy was performed to visualize anisotropic structures, which the criterion for selection were the antimicrobial activity and the agar well diffusion test, and later the accelerated stability test was performed, where the concomitant influence of the antimicrobial activity with the optical properties were evaluated. The major compounds, mainly β-Caryophyllene 32.43%, β-Bisabolene 11.28% and α-Humulene 8.41%. In the disc diffusion test the strains of Gardnerella vaginalis and Staphylococcus epidermitidis were the most sensitive and presented MIC of 500 μg / mL, with bacteriostatic activity. After 24 hours of preparation, all formulations at concentrations of 100 mg/g and 50 mg/g showed homogeneity and anisotropic behavior. In the agar well diffusion test the formulations at concentrations of 100mg / g were higher than 50 mg/g, with a larger number of samples with inhibition halos with significance when compared to the averages of the halos of the oleoresin, especially for the samples at concentrations of 5%, 10% and 15% water. In the accelerated stability test, no relevant physical and anisotropy changes were observed, the most active strains on Day 1 were Gardnerella vaginalis and Pseudomonas aeruginosa and remained the most active until day 60. The sample with 5% water showed to be the most stable, showing no significant decrease of inhibition halos at 25 ° C, but showed a significant decrease in the inhibition halos at 45 ° C from Day 30. It was observed that the sample 15% water was the least stable, showing significant decrease in both the temperature of 25 ° C and 45 ° C. The results indicated the liquid-crystalline formulations potentiated the antimicrobial activity of the ORCR and the concentration of water directly influences the antimicrobial activity and its stability.
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14
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BRENNO DE SOUSA FERREIRA
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Carboxiterapy effects on treatment of cutaneous injuries in rats
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Líder : ADRIANA CAROPREZO MORINI
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MIEMBROS DE LA BANCA :
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ADRIANA CAROPREZO MORINI
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JUAREZ DE SOUZA
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MAXWELL BARBOSA DE SANTANA
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Data: 26-jul-2019
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Resumen Espectáculo
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This study aimed to evaluate the effects of carboxytherapy, infusion in the subcutaneous tissue of medicinal CO2, in the healing of cutaneous lesions in Wistars rats. This is an experimental and quantitative study, with a sample of 10 animals divided into a control group and a carboxytherapy group. All animals suffered a metallic punch injury of 5mm diameter in the dorsal region and the group with therapy treated for 10 consecutive days and then euthanized on the 11th day of the experiment. The region was photographed at various moments of the research to analyze the morphometric evolution of the lesion area and tissue samples containing the lesion were removed for microscopic analysis. . Histological analysis consisted of checking for collagen deposition, proliferation of fibroblasts and new blood vessels. The morphometric results showed that there was no increase in the velocity of closure of the lesion area. The carboxytherapy group presented higher total collagen deposition, type I and type III, and a greater proliferation of fibroblasts compared to the control group, but non-statistically significant data. The evaluation of the formation of new blood vessels had a positive result, with statistically significant numbers (p <0.05). At the end of this research, it was concluded that carboxitherapy may be a good treatment option for cutaneous wounds, especially in lesions with scarring due to microcirculatory deficit.
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15
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SORAIA BAIA DOS SANTOS
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Acute Toxicity Evaluation of Pesticide Clorpirifos in tambaqui (Colossoma macropomum, CUVIER 1818)
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Líder : RUY BESSA LOPES
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MIEMBROS DE LA BANCA :
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RUY BESSA LOPES
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RICARDO BEZERRA DE OLIVEIRA
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FLAVIA GARCEZ DA SILVA
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Data: 06-ago-2019
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Resumen Espectáculo
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Assessing the toxicity of pesticides used in temporary crops for aquatic organisms allows to elucidate newly installed or aggregated disturbances over time. Thus, this ecotoxicological study aimed to evaluate the acute toxicity of the 48% chlorpyrifos pesticide (Klorpan® 480EC) in order to determine the mean lethal concentration (LC50) for tambaqui (Colossoma macropomum) fingerlings. Toxic sensitivity of the test organism was preliminarily assessed using the reference substance “Potassium dichromate”. Acute toxicity tests were performed under controlled laboratory conditions for 96 hours of exposure under static system. The medium weight of juveniles was 1.02 ± 0.06g were distributed in groups of n = 15 formed by triplicates of 5 individuals per aquarium for each concentration of the established lethality range: 2.5; 10; 15; 25 and 45 µg / L, plus one control (white). The lethality data were processed by the Trimmed Spearman-Karber method, which by Probito analyzes determined the LC50-96h of 16.42 µg / L of the chlorpirifos for tambaqui juveniles. Fast and severe effects caused the animals to die within 48h of the test, and behavioral changes were observed up to 96h of exposure. Prior to inducing death in animals, this pesticide caused some behavioral changes at all concentrations, such as: accelerated operculum movement, continuous swimming, surface breathing, loss of swimming balance, erratic swimming, paralysis, spasms, seizures, and others. The Exploratory qualitative / quantitative methodological analyzes of these behavioral effects demonstrated a possible concentration-response relationship. The results suggest that the chlorpyrifos pesticide has been shown to be very toxic to fish and poses a major threat to the survival of aquatic biota.
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16
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HIAGO SOUSA PINHEIRO
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ANTIMICROBIAN USE AND ACUTE KIDNEY INJURY OCCURRENCE INPATIENTS IN A WEST HOSPITAL OF PARÁ
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Líder : WALDINEY PIRES MORAES
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MIEMBROS DE LA BANCA :
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ADJANNY ESTELA SANTOS DE SOUZA
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BRUNO ALEXANDRE DA SILVA
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TANIA MARA PIRES MORAES
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WALDINEY PIRES MORAES
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Data: 22-ago-2019
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Resumen Espectáculo
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Pharmacovigilance is the science of detecting, evaluating, understanding, preventing adverse effects or any drug-related problems. Its main objective is to ensure patient safety and rational use. In studies on adverse drug reactions, among the classes most likely to cause harm to organisms are antimicrobials. This class of drugs may induce nephrotoxicity and cause complications in patients. One of the most common problems with high incidence rates in the hospital setting is acute kidney injury (AKI), a complication that affects most patients and can lead to kidney failure or even death. The aim of this study was to evaluate the incidence of acute kidney injury in patients hospitalized under exposure to nephrotoxic antimicrobials at the Baixo Amazonas Regional Hospital, as well as to describe the demographic and clinical profile of the patients, to verify the variation of serum creatinine during the use of antimicrobials with potential to promote nephrotoxicity and determine plasma vancomycin concentrations. The study was a prospective, observational cohort of hospitalized patients between October 2018 and January 2019. Patients were assessed for inclusion and exclusion criteria on the fifth day of hospitalization. As sources for data collection were used the medical records, prescriptions and results of laboratory tests available in the patient records. Included participants were interviewed and followed for identification of AKI development according to the KDIGO guidelines and plasma quantification of vancomycin concentrations was also performed in patients using this antibiotic. A total of 70 patients were evaluated, with a mean age of 52.49 ± 20.31 years, most of them male (64.29%; n = 45) and coming from the cancer clinic (34.29%; n = 24). Of the patients followed, 48.57% (n = 34) developed AKI and 20.59% (n = 7) reached stage 3 AKI. 20.00% (n = 14) of the patients died, of which seven had developed AKI. An average of 10.26 ± 2.9 drugs prescribed per patient was identified. The most prescribed antimicrobials were ceftriaxone, cefepime and piperacillin/tazobactam. Of the patients using amphotericin B for infectious treatments, 100% (n = 2) presented AKI and 60% (n = 6) of the patients receiving vancomycin antibiotic were diagnosed with AKI. The results of plasma vancomycin concentrations showed that patients who developed AKI during vancomycin use had concentration values ranging from 3 μg/mL to 15.9 μg/mL, most with values below the minimum recommended concentration (10 -20 µg/mL). Understanding the factors associated with the occurrence of AKI in patients admitted to the hospital under exposure to nephrotoxic antimicrobials is of great relevance, as it serves as an aid for the early identification of drug-induced renal dysfunction, providing prevention and appropriate management of these patients, It also reinforces the importance of the pharmacist's role in caring for critically ill patients.
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17
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NAZARÉ CARNEIRO DA SILVA
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IN VITRO ANTIPLASMODIC ACTIVITY AND IN VIVO ANTIMALARIC ACTIVITY OF ESSENTIAL OIL OF Cyperus articulatus L.
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Líder : WALDINEY PIRES MORAES
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MIEMBROS DE LA BANCA :
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ADJANNY ESTELA SANTOS DE SOUZA
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LEONEIDE ÉRICA MADURO BOUILLET
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WALDINEY PIRES MORAES
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Data: 22-ago-2019
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Resumen Espectáculo
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Malaria is a worldwide health problem and is endemic in over 90 countries, with about 212 million cases worldwide. Brazil reported 174,522 cases of malaria in 2017, an increase of 48.11% over 2016. Despite the keen interest in eradicating malaria, the current is not since efficacy is prophylaxis and drug treatment as used forms. for disease control. Delaying the efficacy of control plasma diagnosis, highlighting the need for development of new compounds against this disease. In the context, the present work studied the essential oil obtained from Cyperus articulatus rhizomes (OECA) to investigate its chemical appearance, acute toxicity and antiplasmodic and antimalarial activities in vitro and in vivo, with and without their own use as therapeutic alternative. malaria. The analysis of the chemical composition of the OECA collected in the Tabocal region in the municipality of Santarém, Pará, Brazil, using an Agilent HP-6890 gas chromatograph, in vivo toxicity evaluation in Balb / c mice according to the OECD Guide, was performed. MTT cytotoxicity with WI-26VA-4 cell line, followed by in vitro antiplasmodic activity using Plasmodium falciparum W2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive) strains cultured in red blood cells in 96-well microplate and In vivo antimalarial infection using Balb / c mice infected with approximately 106 P. berghei-parasitized erythrocytes receiving treatment on the 4th day after inoculation were treated for 7 consecutive days and subjected to blood collection to determine hematological parameters. Chemical characterization by GC-MS allowed the identification of 37 compounds with mustacone as the major compound. The acute toxic dose of OECA is greater than 2000 mg / kg in Balb / c mice, classifying it in category 5 of the Globally Harmonized Classification System (GHS). In vitro, OECA presents low cytotoxicity and high antiplasmodic potential (IC50 <10 µg ml-1) against the two P. falciparum strains tested. In vivo, it significantly reduced (p <0.001) P. Berguei-induced parasitemia and consequently improved hematological parameters at doses of 100 and 200 mg / kg / day. Given the above, OECA represents a product extracted from the Amazon with antiplasmodic and antimalarial potential validating popular use and making OECA a safe and promising candidate for a drug or source of substances, and further studies are needed.
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18
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RÔNISON SANTOS DA CRUZ
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Environmental perception of chemical risk and occurrence of toxic metals in Iripixi Lake, Oriximiná-PA
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Líder : RUY BESSA LOPES
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MIEMBROS DE LA BANCA :
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RUY BESSA LOPES
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PAULO SERGIO TAUBE JUNIOR
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CHARLES HANRY FARIA JUNIOR
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Data: 30-ago-2019
Ata de defesa assinada:
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Resumen Espectáculo
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One of the central aspects of the discussion of natural resource pollution stems from the increasing exposure of natural and human environments to nonpolar chemical compounds such as toxic metals and pesticides. In the municipality of Oriximiná- PA due to urban expansion and agricultural activities on the shores of Lake Iripixi, ecotoxicological studies can be used as instruments to defend environmental and human health. The objective of this work was to evaluate the environmental perception of the residents around Lake Iripixi regarding pesticide exposure and toxic metal occurrences in relation to the environment and public health. Data collection was done by applying a semi-structured questionnaire, closed and open. For the analysis of toxic metals, in this study we adopted the protocol Sediment Quality Assessment Directive (DAQS). The DAQS evaluated the degree to which the chemical status of the toxicant when associated with sediment adversely affects aquatic organisms. Multivariate analyzes of toxic metal content in sediments were performed. Pearson correlation analysis, factor analysis (FA) and hierarchical cluster analysis were also performed. According to the data, the description of the environmental problems listed by the residents around the Iripixi lake, Oriximiná-PA, were; 50% sewage; silting 26.08%; deforestation 2.17%; pesticide 4.34%; eutrophication 2.17% and another 15.21%. Lack of sanitation is the biggest environmental problem in the region where they live. Toxic metal contents Al, Cd, Cr, Cu, Ni, Pb and Zn are within the proposed reference values (VR) 1 for unpolluted surface waters and sediment in the Amazon. Important strategies must be implemented to reduce the discharge of domestic effluents; such as controlling non-point agricultural pollution to reduce the environmental risks associated with toxic metals.
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19
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ADRIELLE LEAL DIAS
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Evaluation of Epidemiological and Clinical Factors related to Adverse Reactions to Polychemotherapy in Leprosy Patients in the Municipality of Santarém-Pará.
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Líder : GUILHERME AUGUSTO BARROS CONDE
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MIEMBROS DE LA BANCA :
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GUILHERME AUGUSTO BARROS CONDE
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MARIA GORETH SILVA FERREIRA
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Nádia Vicência do Nascimento Martins
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Data: 02-sep-2019
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Resumen Espectáculo
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Leprosy known as lepro is one of the oldest reported endemics. It is an infectious disease, chronic evolution, compulsory notification, curable and the mandatory investigation throughout the national territory. It is characterized by its natural evolution that leads to physical disability due to damage of peripheral nerve trunks due to the invasion of neural cells caused by Mycobacterium leprae and the body's response to the infectious process. This study aimed to evaluate the factors related to cases of adverse reactions and leprosy treated with multidrug therapy diagnosed from 2007 to 2017 in the city of Santarém-PA. For this, 95 medical records from 10 Health Units of the Municipality of Santarém -PA from 2007 to 2017 were evaluated. After the evaluation of the epidemiological and clinical variables proposed in the methodology, it was observed that the reaction patients in this sample were characterized by male predominance. associated with a higher risk of development of the multibacillary form, predominantly presenting the dimorph clinical form, with development of more frequent leprosy reactions during treatment corroborating with most of the studies found in the literature. Regarding adverse reactions, the records were mostly in females, with the clinical form remaining dimorph. According to our evaluations of the available records, abandonment cases are not directly related to those with leprosy reactions and adverse reactions, although some studies in the literature associate certain clinical and epidemiological variables as determining factors, especially for treatment abandonment cases.
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20
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MAYARA DUARTE DA SILVA
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PUBLIC AND PRIVATE IN HIGH SCHOOL IN SANTARÉM-PARÁ: WHAT IS THE DIFFERENTIAL OF COGNITIVE PERFORMANCE?
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Líder : PAULO SERGIO TAUBE JUNIOR
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MIEMBROS DE LA BANCA :
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DOMINGOS LUIS WANDERLEY PICANCO DINIZ
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ELDRA CARVALHO DA SILVA
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JOANILSON GUIMARAES SILVA
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Data: 22-nov-2019
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Resumen Espectáculo
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The need to increase the supply of Brazilian schooling was not accompanied by a concern with the quality of education offered. The first actions aimed at the implementation of a national basic education evaluation system in Brazil began in the 1980s, with the implementation of several educational reforms. During this period, Brazilian educational research was strongly influenced by the Coleman Report published in 1966 in the United States. This research has given new directions to both educational assessments and the allocation of social capital, revealing that education is part of a set of factors, and that research in the area needs to address social and school factors in educational inequalities. Against with a growing demand for measures that reverse the insufficient levels of learning revealed by the current evaluations of Brazilian basic education, we evoke here a discussion about the idea of education as a foundation for better individual formation and, thus, for the construction of a society fairer. The purpose of this paper was to study the cognitive performance profile for high school students in two schools, a Public School - EPU and a Private School - EPR, from the urban area of Santarém-Pará, from 2011 to 2017. This is a quantitative and qualitative research, with secondary data collection conducted from October 2018 to September 2019, with permission to consult the annual final results maps by the directors of the participating educational institutions, as well as from available data by the official national websites. The study showed significant differences in cognitive performance between the two schools, the EPU showed greater oscillations in performance averages compared to the EPR. The EPU proved to be the institution with the highest red scores, disapprovals, cancellations, retentions and abandonmet in the period evaluated. The EPU students had more difficulty adapting to high school than EPR students, suggesting that these students progress to high school with a poor elementary school background, a fact reproduced over the years with no reversal measure, reflecting inequalities between the performances diagnosed in the two schools. The number of enrollments, as well as the number of students per class is much higher at EPU than EPR, which reflects the higher Indicator of Teaching Effort in High School, suggesting that the quality of education may also be correlated with these variables. The research also suggests that the most important factor in
determining teaching efficiency in the two institutions evaluated is more correlated to didactic- pedagogical, administrative, and extra-school factors, such as family aspects, i.e., than material
or infrastructure inputs, being necessary further studies aimed at better characterization of these results. Thus, the collective construction of a path of participation and co-responsibility for improving the quality of public education may be: a greater emphasis on the basis of teacher education; and greater attention to deficiencies in primary education.
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21
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MAYARA DUARTE DA SILVA
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PUBLIC AND PRIVATE IN HIGH SCHOOL IN SANTARÉM-PARÁ: WHAT IS THE DIFFERENTIAL OF COGNITIVE PERFORMANCE?
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Líder : PAULO SERGIO TAUBE JUNIOR
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MIEMBROS DE LA BANCA :
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DOMINGOS LUIS WANDERLEY PICANCO DINIZ
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ELDRA CARVALHO DA SILVA
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JOANILSON GUIMARAES SILVA
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SIANY DA SILVA LIBERAL
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Data: 22-nov-2019
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Resumen Espectáculo
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The need to increase the supply of Brazilian schooling was not accompanied by a concern with the quality of education offered. The first actions aimed at the implementation of a national basic education evaluation system in Brazil began in the 1980s, with the implementation of several educational reforms. During this period, Brazilian educational research was strongly influenced by the Coleman Report published in 1966 in the United States. This research has given new directions to both educational assessments and the allocation of social capital, revealing that education is part of a set of factors, and that research in the area needs to address social and school factors in educational inequalities. Against with a growing demand for measures that reverse the insufficient levels of learning revealed by the current evaluations of Brazilian basic education, we evoke here a discussion about the idea of education as a foundation for better individual formation and, thus, for the construction of a society fairer. The purpose of this paper was to study the cognitive performance profile for high school students in two schools, a Public School - EPU and a Private School - EPR, from the urban area of Santarém-Pará, from 2011 to 2017. This is a quantitative and qualitative research, with secondary data collection conducted from October 2018 to September 2019, with permission to consult the annual final results maps by the directors of the participating educational institutions, as well as from available data by the official national websites. The study showed significant differences in cognitive performance between the two schools, the EPU showed greater oscillations in performance averages compared to the EPR. The EPU proved to be the institution with the highest red scores, disapprovals, cancellations, retentions and abandonmet in the period evaluated. The EPU students had more difficulty adapting to high school than EPR students, suggesting that these students progress to high school with a poor elementary school background, a fact reproduced over the years with no reversal measure, reflecting inequalities between the performances diagnosed in the two schools. The number of enrollments, as well as the number of students per class is much higher at EPU than EPR, which reflects the higher Indicator of Teaching Effort in High School, suggesting that the quality of education may also be correlated with these variables. The research also suggests that the most important factor in
determining teaching efficiency in the two institutions evaluated is more correlated to didactic- pedagogical, administrative, and extra-school factors, such as family aspects, i.e., than material
or infrastructure inputs, being necessary further studies aimed at better characterization of these results. Thus, the collective construction of a path of participation and co-responsibility for improving the quality of public education may be: a greater emphasis on the basis of teacher education; and greater attention to deficiencies in primary education.
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22
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PAULO SERGIO FERREIRA DE LIMA
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DESIGN, SYNTHESIS AND EVALUATION OF THE ANTI-INFLAMMATORY ACTIVITY OF METHYL NITRO-SALICYLATE IN VITRO IN MACROPHAGOS
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Líder : WALDINEY PIRES MORAES
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MIEMBROS DE LA BANCA :
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WALDINEY PIRES MORAES
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BRUNO ALEXANDRE DA SILVA
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JUAREZ DE SOUZA
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Data: 17-dic-2019
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Resumen Espectáculo
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Inflammation is a series of protective and regenerative responses from the human body, characterized by a series of events involving inflammatory mediators such as cytokines and cells, being classified as acute or chronic inflammation. Throughout this process are involved leukocyte migration, recognition of PAMPs and DAMPs by TLRs receptors by tissue macrophages, production of nitric oxide, cytokines and eicosanoids such as PGE2. Non-steroidal anti-inflammatory drugs (NSAIDs) appear among the most consumed and their action is due to the mechanism of inhibition of COX enzymes, corticosteroids are drugs that have anti-inflammatory effects with mechanism of action associated with binding with glucocorticoid receptors, cytosolic, regulating gene expression of immune cells. About salicylates, they have anti-inflammatory effects, as well their derivatives, on the other hand, have a number of adverse reactions from which we can mention the gastrointestinal, renal and cardiovascular problems. The creation of new molecules has become an art for scholars in the field, seen to the need correlated the pharmaceuticals industries, techniques like these are important for the development of new pharmaceuticals products such as those used for salicylates. NSAIDs have proven toxicity and are considered harmful to the renal and gastrointestinal and cardiovascular systems, therefore, methyl salicylate derivatives, with their anti-inflammatory effects, are an option in the development of new drugs to be used to combat inflammation. The aim of this study is to design, synthesize and evaluate the anti-inflammatory activity of Methyl Nitro-Salicylate in vitro in macrophages. To obtain the NSM, we started from a reaction performed between three compounds, the assembly and production of this new drug were executed in partnership with the Pharmaceutical Chemistry Laboratory of the Federal University of Pará. Cell viability was performed by the MTT method, the production of NO was by the Griess method, the dosages of IL-1β, TNF-α and PGE-2 were quantified by the ELISA method. As result, the reaction performed between three compounds resulted in the formation of Methyl Nitro-Salicylate. NSM did not promote cytotoxic effect on concentrations below 300 μM after 24 hours of incubation, causing cell death at a concentration of 1000 μM. Regarding the tests of NO, TNF-α, IL-1β and PGE2, the results showed that NSM promoted a significant reduction in the production of these inflammatory mediators in the groups simulated with LPS + INF-γ and treated with concentrations of 12.5, 25 and 50 µM where it was possible to observe a concentration dependent effect. However, NSM has been shown to have a potential anti-inflammatory effect, however, further studies should be conducted in order to identify the mechanism of action by which NSM can exert its anti-inflammatory effect.
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23
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MARCELLO DE LIMA BAIMA
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BURNOUT SYNDROME AND NUTRITIONAL PROFILE OF WEST FEDERAL UNIVERSITY PROFESSOR
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Líder : WALDINEY PIRES MORAES
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MIEMBROS DE LA BANCA :
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WALDINEY PIRES MORAES
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MARINA SMIDT CELERE MESCHEDE
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TANIA MARA PIRES MORAES
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Data: 17-dic-2019
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Resumen Espectáculo
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Burnout syndrome (BS) is a chronic stress process, which begins with prolonged levels of stress and pressure at work. The public higher education teacher is subjected to several stressors that may cause BS, such as: the entrance through a contest; periodic evaluations and need for training for professional progressions; excessive workload; performance in teaching, research and extension; deal with inexperienced students, fresh out of a completely different educational structure. Chronic stress causes systemic changes that alter body homeostasis, including the regulation of food intake, which can cause changes in the nutritional status of the individual. Objectives: to identify and analyze Burnout Syndrome; trace the nutritional profile; correlate BS to the nutritional status of the Federal University of Western Pará faculty. Methods: A cross-sectional, non-randomized study was conducted with faculty members who entered the institution until December 2017. To identify the BS, the “Cuestionario para la Evaluación del Síndrome Quemarse por el Trabajo”- CESQT, for teachers, adapted and validated for Portuguese. The instrumental is divided into four dimensions: illusion at work (IW), psychic burnout (PB), indolence (IND) and guilt (G), where low scores on IL and high scores on PB, IND and G are indicative of BS. To determine the nutritional status, anthropometric assessment and electrical bioimpedance examination were performed. The parameters used were Body Mass Index (BMI), Fat Percentage (%BF) and Visceral Fat Area (VFA). The research project was approved by CEP/FSCMPA. Results: Forty-four teachers participated in the research, 29 (65.9%) female and 14 (34.1%) male, with a mean age of 41.0 (± 8.33). It was found that 33 (75%) teachers had scores indicating burnout in at least one dimension of CESQT, with the PB being more frequent, 65.9% of the sample. Regarding nutritional status, 51.4% were obese and overweight as regards BMI; 77.8% had higher body fat percentage than indicated and 47.2% had VFA above the reference value. Conclusion: It was found in the sample the high incidence of burnout syndrome among participants, but classified as low intensity, these results can be explained by the fact that many of them are in their first and still little teaching experience. Regarding nutritional status, it was observed that more than half of the teachers presented inadequacy regarding BMI and %BF, associated with overweight and high body fat percentage. There was no statistical correlation between BS and change in nutritional status.
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