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Microbial ecology. Gene mcrA. Gene pmoA. Methanotrophic. Methanogenic.
Methane is a colorless, odorless gas with little water solubility, has great energy potential and comes from the decomposition of organic matter. Together with carbon dioxide, methane is a major contributor to the greenhouse effect. In this sense, the Amazon has an important role in the global CH4 cycle, as it presents a significant reservoir of this gas in flooded regions. This work aims to characterize the community and the diversity of microorganisms Archaea and Bacteria related to the production and oxidation of methane gas (CH4) from sediment samples collected in lowland areas in the Arapixuna community, located in the municipality of Santarém-PA, in periods of drought and flood, by sequencing the 16S, mcrA and pmoA genes. To estimate the interactions between microbial diversity and the methane cycle, molecular analyzes, methane flow measurements, physical-chemical analyzes of the soil and the richness and diversity coefficient will be combined. Thus, it is expected to identify the main metanotrophic and methanogenic microorganisms present in the lowland soil of the Arapixuna region and to correlate their presence with the methane indexes estimated for the area.