Banca de QUALIFICAÇÃO: ISADORA ELAINE SILVA DA CRUZ

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ISADORA ELAINE SILVA DA CRUZ
DATE: 15/07/2022
TIME: 09:00
LOCAL: https://meet.google.com/sea-hmfr-yvo
TITLE:

IDENTIFICATION OF BENTHONIC MACROINVERTEBRATES PRESENT IN AMAZON VARZEAS THROUGH ENVIRONMENTAL DNA

KEY WORDS:

Benthic macroinvertebrates. Floodplains. environmental DNA.

PAGES: 31
BIG AREA: Outra
AREA: Ciências Ambientais
SUMMARY:

A new approach called metabarcoding for environmental DNA has grown considerably in recent years, this method aims to identify the diversity of taxa and the composition of biodiversity, using universal primers. With the aid of this technique, the objective of this research is to identify benthic invertebrates in two Amazonian floodplains in the wet and dry periods of 2022/2023. The sediment and water samples used in this work will be collected in the floodplains of Pixuna do Tapará and Arapixuna, municipality of Santarém-PA. Immediately after collection, the water samples will be filtered. After the DNA extraction, we will perform the sequencing of the Cytochrome Oxidase C subunit I (COI) gene, a region that is most used in DNA metabarcoding studies of macroinvertebrates. All steps of data analysis will be performed in R 4.5.0 software. By using the environmental DNA technique to analyze the diversity of macroinvertebrates, it is expected to understand the role that Amazonian floodplain regions have in the possible survival strategies of these organisms. Finally, with these results we can obtain crucial information about the presence or absence of these organisms in these environments and their interaction with different abiotic factors of the floodplain ecosystem.

BANKING MEMBERS:
Presidente - 1987988 - GABRIEL IKETANI COELHO
Interno - 1327077 - LUIS REGINALDO RIBEIRO RODRIGUES
Externa à Instituição - TALITA FERNANDA AUGUSTO RIBAS - UFOPA