Banca de DEFESA: FERNANDA CRISTINA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FERNANDA CRISTINA DOS SANTOS
DATE: 25/10/2022
TIME: 08:00
LOCAL: https://meet.google.com/ewr-ciip-auw
TITLE:

DEVELOPMENT OF MOLECULAR DIAGNOSTICS FOR THE ETIOLOGICAL AGENTS OF CASSAVA ROOT ROOT IN THE WESTERN REGION OF PARÁ

KEY WORDS:

Manihot esculenta, Molecular diagnosis, Etiology, Diversity

PAGES: 38
BIG AREA: Outra
AREA: Ciências Ambientais
SUMMARY:

Root rots are diseases caused by a complex of fungi, which in cassava have a continuous and significant impact on production and consequently cause socioeconomic damage to producing countries. For disease control, the correct diagnosis of the pathogen is essential. Thus, the objective of this work was to develop a molecular tool to differentiate the causal agents of cassava root rots through the ITS-RFLP of the main causal agents known in Brazil (Fusarium solani, Neoscytalidium dimidiatum, Phytophthora melonis, Phytopythium sp. and Lasiodiplodia theobromae) digested by selected enzymes in silico (EcoRI, BmgBI, HaeIII, SmaI and XhoI). It was possible to verify the differentiation of the phytopathogens in two groups (ascomycetes and oomycetes), only by the polymorphism of the fragment size of the ITS region. Furthermore, it was possible to differentiate dry rot from black rot using the enzymes HaeIII, SmaI and EcoRI, as well as, within the oomycetes, to differentiate the species (P. melonis, Phytopythium sp.) through the enzymes BmgBI and XhoI. Therefore, the ITS-RFLP can be a new molecular tool for the rapid and efficient diagnosis of the causal agents of cassava root rot in the main producing regions.

COMMITTEE MEMBERS:
Externa à Instituição - ALESSANDRA KEIKO NAKASONE - EMBRAPA
Presidente - 1966924 - CARLOS IVAN AGUILAR VILDOSO
Externo à Instituição - SAULO ALVES SANTOS DE OLIVEIRA - EMBRAPA