Banca de DEFESA: SARA FREITAS DE SOUSA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : SARA FREITAS DE SOUSA
DATE: 20/01/2022
TIME: 08:00
LOCAL: Videoconferência - Link: https://meet.google.com/crk-hxgx-zmi
TITLE:

Phytochemical, antioxidant and biological activity studies of constituents of woody waste and sap of Hymenaea courbaril.

KEY WORDS:

Jatoba. Flavonoids. Antimicrobial activity.

PAGES: 139
BIG AREA: Outra
AREA: Ciências Ambientais
SUMMARY:

The Fabaceae family is widely distributed which Hymenaea courbaril is one of the most important species. Because of its multiple use and ethnopharmacological and pharmacological potential, it is crucial to investigate its chemical constitution and bioactive compounds. In this study, we aimed to perform the phytochemical analysis, antioxidant and antimicrobial activity of the sap and ethanolic extract of H. courbaril wood residues. Forest residues and sap were obtained from an Amazonian native forest managed by local foresters near
the city of Santarém, Pará, Brazil. The sawdust was oven-dried at 40°C for 96 h and extraction of secondary
metabolites was performed with ethanol in a Soxhlet apparatus. The following techniques were performed to
complete phytochemical analysis: Thin Layer Chromatography (TLC), Gas Chromatography coupled to Mass
Spectrometry (GC-MS), High Performance Liquid Chromatography coupled to a photo-diode array detector (HPLCPDA), High Performance Liquid Chromatography coupled to a Mass Spectrometer (HPLC-ESI-MS/MS), using the molecular interaction networks approach or molecular networking through the GNPS (Global Natural Products Social Molecular Networking) platform. One and two dimensional Nuclear Magnetic Resonance technique, (1H, 13C, HMBC, HSQC) was used for the identification of the isolated substances. The antioxidant activity (DPPH and FRAP), quantification of total flavonoids, phenolic compounds contents were done by spectrophotometric methods. The antimicrobial activity of both extract and sap of H. courbaril were performed against microorganisms of clinical interest by the minimum inhibitory concentration (MIC) technique. The ethanolic extract has a higher antioxidant potential and has the highest content of phenolic compounds and flavonoids when compared to the sap. The predominant compound class in both the extract and the sap are flavonoids, which enabled identification of 16 substances including taxifolin derivatives through spectral correspondence with experimental data from GNPS. Through the NMR technique it was possible to isolate and identify the substances fustin, taxifolin, 3-methoxy-quercetin and quercetin. The molecules identified and isolated in this work are highly important for this species, and may be the reason for its bioactivity. Thus, this study contributes to the traditional safe use of H. courbaril, and, in the long term, it may result in production of herbal medicines and formulations.

BANKING MEMBERS:
Externo à Instituição - DERCILIO JUNIOR VERLY LOPES
Interno - 1843993 - ANTONIO HUMBERTO HAMAD MINERVINO
Externo à Instituição - ISABELLA CRISTHINA GONÇALVES COSTA
Presidente - 152.758.687-15 - LAURO EUCLIDES SOARES BARATA - UFOPA
Externo à Instituição - RAPHAEL CARLOS FERRER DE SANTANA
Interno - 1795661 - VICTOR HUGO PEREIRA MOUTINHO