Banca de DEFESA: BRUNA CRISTINE MARTINS DE SOUSA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : BRUNA CRISTINE MARTINS DE SOUSA
DATE: 28/07/2022
TIME: 14:30
LOCAL: Sala Virtual
TITLE:

Chemical composition and biological activity of plant extracts of Dipteryx punctata (S.F. Blake) Amshoff from Mojuí dos Campos, Pará

KEY WORDS:

Cumaru; plant extracts; Phytochemistry; antioxidant activity; alternative control.

PAGES: 34
BIG AREA: Outra
AREA: Ciências Ambientais
SUMMARY:

Dipteryx spp. belong to the Fabaceae family, and their neotropical trees are native to Central and South American countries. Among the species of this genus, Dipteryx odorata stands out historically in the Amazon, for the commercialization of its seeds and extraction of coumarin, the main active compound, and Dipteryx punctata lacks studies that detail its botanical description, phytochemical composition and biological activities. In this sense, this work aimed to evaluate the chemical profile and the antioxidant and antifungal activities of ethanolic extracts of D. punctata. A systematic review was carried out on the genus Dipteryx using SciELO and the Brazilian Digital Library of Theses and Dissertations (BDTD) as databases. For chemical and biological evaluations, plant material was collected in five areas producing cumaru seeds, in Mojuí dos Campos, Pará. The extracts were obtained via Soxhlet, using 92.8% distilled ethanol as solvent, with a total duration of eight hours, calculating the final yield. Chemical analyzes of the extracts were performed by Thin Layer Chromatography (TLC), Gas Chromatography coupled to Mass Spectrometry (GC-MS), and determination of phenolics and flavonoids. Antioxidant activity was performed by scavenging free radicals DPPH (2,2-diphenyl-1-picryl-hydrazyl). The antifungal activity of the extracts was evaluated under in vitro and in vivo conditions. In the in vitro assay, the extracts and the coumarin standard were tested at concentrations: 10%, 20%, 30%, 40% and 50% (p: v), added in potato-dextrose-agar (PDA) medium, over the fungus Colletotrichum musae, the causal agent of anthracnose disease in bananas. The control consisted of fungal growth only in PDA medium. The experimental design was completely randomized (DCR), in a factorial scheme, with four replications. The evaluations were carried out by measuring the average diameter of the colonies, daily, for eight days. With the data from the colonies, the percentage of inhibition of growth (PIG), mycelial growth velocity index (MGVI) and the growth rate (GR) were determined. In the in vivo assay, the extracts and coumarin were tested at concentrations of 40% and 50%, in preventive and curative applications. The experimental design was completely randomized (DCR), in a factorial scheme, with three replications. The evaluations were carried out by measuring the average diameter of the lesions at intervals of two days, up to eight days after inoculation. The data obtained in the antifungal assays were submitted to analysis of variance and compared by the Tukey test (p≤0.05), using the statistical software SISVAR 5.6. Studies on the genus Dipteryx are more restricted to the species Dipteryx alata and Dipteryx odorata. The highest yields of extracts were obtained for residues and fruit seeds. The extracts from leaves, branches, residues and seeds showed terpenes, condensed and hydrolysable tannins, flavonoids and coumarin, as well as strong antioxidant action. The extracts from the branches and residues of the fruits of D. punctata and the coumarin pattern, caused the greatest inhibitions in the growth of the phytopathogen in vitro and in vivo, all extracts were effective, especially coumarin and preventive applications of concentrations tested. The results obtained for the extracts are promising and sources of investigation for the future development of natural products aimed at controlling anthracnose in a more sustainable way.

BANKING MEMBERS:
Presidente - 1776327 - THIAGO ALMEIDA VIEIRA
Interno - 1693122 - SERGIO DE MELO
Interno - 2143267 - JOSE MAX BARBOSA DE OLIVEIRA JUNIOR
Externa ao Programa - 1964235 - HELIONORA DA SILVA ALVES
Externa ao Programa - 1782945 - DENISE CASTRO LUSTOSA
Externa à Instituição - LYVIA JULIENNE SOUSA REGO
Externo à Instituição - ROGÉRIO EIJI HANADA - INPA