GENOTOXIC AND HISTOLOGICAL EVALUATION IN FISH (Hyphessobrycon heterorhabdus) OF IGARAPÉ CARARAZINHO EXPOSED TO CHORUM FROM LANDFILL DUMP SANTARÉM - PA.
Micronucleus; comet assay, Histopathology
The enormous waste production is one of the major environmental issues in the present. The waste inappropriate discard and management give serious risks to the human and ecosystem health. The leachate, liquid produced by garbage decomposition, can contain many molecules potentially damaging to genetic material, such as heavy metals and aromatic polycyclic hydrocarbon (APH). The present study goals to evaluate the genotoxic and histopathological effects of the leachate from Santarém Landfill Dump (Lixão do Perema) in fishes Hyphessobrycon heterorhabdus. Micronucleus, erythrocyte nuclear abnormalities techniques and comet assay were conducted to genotoxicity evaluation, while tissue injuries were assessed with gills and liver histological preparations. The fishes were exposed to leachate (10, 5 and 2%), to natural stream Cararazinho’s water and to 20ppm benzene. The genotoxicity biomarkers do not evidenced significant variation between exposed and control group (stream natural water). Gills and liver showed diverse types of tissue lesions. Therefore, under the light of these results we conclude that Cararazinho’s stream water, because receives leachate from the Santarém Landfill Dump, plays to the occurrence of genotoxic and tissue damages in fishes H. heterorhabdus.