Banca de DEFESA: JOSIANE ELIZABETH ALMEIDA E SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOSIANE ELIZABETH ALMEIDA E SILVA
DATA : 28/06/2019
HORA: 15:00
LOCAL: LABORATÓRIO DE BIOTECNOLOGIA VEGETAL
TÍTULO:

CHEMICAL CHARACTERIZATION, MICROBIOLOGICAL EVALUATION AND DEVELOPMENT OF A FORMULATION WITH Copaifera reticulata Ducke OIL

PALAVRAS-CHAVES:

Copaifera, chemical composition, antibacterial activity, formulation.

PÁGINAS: 52
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biotecnologia
SUBÁREA: Biotecnologia Vegetal
ESPECIALIDADE: Tecnologia de Processos e Produtos
RESUMO:

The biological properties of medicinal plants are attributed to their chemical potential which performs some activities already described in the literature. Among them is the antimicrobial activity, which has been studied due to the increase of bacterial resistance to multiple antimicrobial drugs. In view of the demand for new therapeutic alternatives, medicinal plants represent an important source for obtaining these medicines. Copaiba oleoresin has several proven medicinal properties, among them antimicrobial activity. Therefore, the aim of this work is to evaluate the chemical composition, cytotoxicity and microbiological activity against strains Staphylococcus aureus; S. aureus resistant to methicillin and S. simulans in different seasonal periods (dry and rainy), as well as to develop a semi-solid phytotherapeutic formulation of the oleoresin (in natura) of Copaifera reticulata Ducke. This work carried out trials that evaluated the chemical composition of oleoresin by GC-MS, cytotoxic activity in vitro using VERO cells, the microbiological activity by well diffusion and microdilution in plates and developed a semi-solid formulation using an emulsified system. In relation to the results found, it was possible to identify  (2S and C, 3S and C), in the chemical analysis that β-caryophyllene was the major component in all the samples used, samples 2C, 2S, 3C and 3S presented cytotoxicity only at the concentration of 200 μg / mL, and the sample 1 C and 1 S did not show cytotoxicity at any of the concentrations used, in all the bacteria used found inhibition halos indicating that there was action of the oleoresin. The determination of the Minimum Inhibitory Concentration (MIC) showed that the oleoresin collected during the rainy season showed strong inhibition, whereas in the dry period it showed a weak inhibition. The formulation incorporated with the oleoresin was stable, gel-like, light yellow in color and woody odor, with appearance of anisotropy and birefringence, with texture in the form of striations, characteristic of hexagonal phase that has been used in some studies to sustained release of Drugs. The results obtained in the present work reinforce even more the importance of copaíba oleoresin from the Amazon region as a natural herbal medicine with antibacterial potential, being thus a promising source for the elaboration of new antimicrobial drugs.

 

MEMBROS DA BANCA:
Presidente - 1776289 - ELAINE CRISTINA PACHECO DE OLIVEIRA
Interna - 2139720 - KARIANE MENDES NUNES
Externa ao Programa - 1833953 - SILVIA KATRINE SILVA ESCHER