Banca de DEFESA: VICTOR HUGO RABELO DE AQUINO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : VICTOR HUGO RABELO DE AQUINO
DATA : 12/07/2019
HORA: 15:00
LOCAL: Auditório do NTB
TÍTULO:

DEVELOPMENT OF BUTTER-BASED PHYTOTERAPIC VAGINAL GEL OF Astrocaryum murumuru Mart.CONTAINING OLEORRESIN OF Copaifera reticulata Ducke FOR THE TREATMENT OF VAGINOSIS

PALAVRAS-CHAVES:

Copaifera reticulata, Astrocaryum murumuru, Liquid crystal, Antimicrobial activity, Accelerated stability.

 

PÁGINAS: 22
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biotecnologia
SUBÁREA: Biotecnologia em Saúde Humana e Animal
RESUMO:

The copaiba is a plant widely used in folk medicine, studies have evidenced several biological activities, mainly antimicrobial. The use of natural products becomes an effective and economical alternative to avoid the inappropriate use of antibiotics. The search for new drug delivery systems has been of great relevance in order to make the therapy more efficient, among these release systems the liquid crystals stand out due they show some technological advantages, promoting greater stabilities against extrinsic factors and allows sustained release of drugs and / or actives. The objective of this study was to develop an phytotherapic antimicrobial gel gel of Astrocaryum murumuru Mart. butter containing Copaifera reticulata Ducke. oil-resin. The Copaifera reticulata oleoresin was collected in the Tapajós National Forest, and its chemical composition was analyzed by Gas chromatography mass spectrometry (GC-MS). The oleoresin antimicrobial activity was performed by the disc diffusion technique, using standard ATCC strains of bacteria related to vaginosis and
evaluated the minimum inhibitory concentration (MIC). Five groups of emulsified systems were obtained from the fixed amount of the Procetyl surfactant with different concentrations of water and murumuru butter, with different concentrations of incorporated oleoresin. After 24 hours of preparation of the formulations, polarized light microscopy was performed to visualize anisotropic structures, which the criterion for selection were the antimicrobial activity and the agar well diffusion test, and later the accelerated stability test was performed, where the concomitant influence of the antimicrobial activity with the optical properties were evaluated. The major compounds, mainly β-Caryophyllene 32.43%, β-Bisabolene 11.28% and α-Humulene 8.41%. In the disc diffusion test the strains of Gardnerella vaginalis and Staphylococcus epidermitidis were the most sensitive and presented MIC of 500 μg / mL, with bacteriostatic activity. After 24 hours of preparation, all formulations at concentrations of 100 mg/g and 50 mg/g showed homogeneity and anisotropic behavior. In the agar well diffusion test the formulations at concentrations of 100mg / g were higher than 50 mg/g, with a larger number of samples with inhibition halos with significance when compared to the averages of the halos of the oleoresin, especially for the samples at concentrations of 5%, 10% and 15% water. In the accelerated stability test, no relevant physical and anisotropy changes were observed, the most active strains on Day 1 were Gardnerella vaginalis and Pseudomonas aeruginosa and remained the most active until day 60. The sample with 5% water showed to be the most stable, showing no significant decrease of inhibition halos at 25 ° C, but showed a significant decrease in the inhibition halos at 45 ° C from Day 30. It was observed that the sample 15% water was the least stable, showing significant decrease in both the temperature of 25 ° C and 45 ° C. The results indicated the liquid-crystalline formulations potentiated the antimicrobial activity of the ORCR and the concentration of water directly influences the antimicrobial activity and its stability.

MEMBROS DA BANCA:
Presidente - 2139720 - KARIANE MENDES NUNES
Externa ao Programa - 2375623 - GABRIELA BIANCHI DOS SANTOS
Externo à Instituição - EDILENE GADELHA DE OLIVEIRA, - UFRGS