Banca de DEFESA: DANIELLE CRISTINE DA CRUZ LOPES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : DANIELLE CRISTINE DA CRUZ LOPES
DATA : 18/12/2020
HORA: 14:30
LOCAL: Ambiente Virtual link: https://meet.google.com/fsn-wupp-tks
TÍTULO:

EVALUATION OF HYPOGLYCEMIC ACTIVITY IN MICE OF THE ESSENTIAL OIL OF PIPER MARGATATUM Jacq. 

PALAVRAS-CHAVES:

Keywords: Hypoglycemic activity. Diabetes. Piperaceae.

PÁGINAS: 83
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
SUBÁREA: Farmacologia Bioquímica e Molecular
RESUMO:

Diabetes Mellitus is a chronic condition that grows progressively, especially in developing countries. 

Considering the growing number of diabetes cases, high costs related to its control, the treatment of its complications, numerous side effects caused by oral and injectable medication and popular use of plants, this study aimed to evaluate the hypoglycemic activity of essential oil of leaves of Piper marginatum in mice. 

This project was submitted to the Ethics Committee on the Use of Animals at UFOPA - CEUA / UFOPA and was approved under protocol n°. 1120180048/2019. 

In the evaluation of acute oral toxicity, 12 female mice, isogenic from the Swiss strain, were submitted to the experiment, which were divided into a control group and a test group in which they received the essential oil of Piper marginatum leaves in a single concentration of 2000mg / kg and observed by 14 days, following the protocol OECD 423/2001. 

To verify the action of the Piper marginatum OE on glycemic levels, 36 male Swiss mice were used and concentrations of 100 mg / kg, 200 mg / kg and 400 mg / kg administered by gavage for 20 days. 

Blood glucose, water and feed intake, body weight and biochemical parameters were evaluated.

The OE was obtained by hydrodistillation in Clevenger apparatus and its chemical characterization by gas chromatography coupled to Mass Spectrometry (GC-MS). 

In the chemical composition, a total of forty-three compounds were identified, the major ones being: 3-carene (13.28%), followed by karyophylene (12.57%), copaene (9.50%), 3,4-methylenedioxypropiophenone (7.96%), elixene (6.02%) and β-cubebene (5.60%).

Regarding acute oral toxicity, the mice showed body weight gain and absence of symptoms or clinical signs of intoxication and death. 

In the glycemic evaluation, it was demonstrated that the OE of Piper marginatum leaves does not show antidiabetic activity because it maintains hyperglycemia in the mice treated with the vegetable until the end of the experiment. 

In addition, during the 20 days the mice showed polydpsy, polyphagia and weight loss. 

In the biochemical assessment, aspartate aminotransferaseand creatinine did not present significant changes, except for urea, which presented values 2.2 times higher in relation to the non-diabetic control group. 

The hepatic marker alanine aminotransferase of the animals treated with the OE of Piper marginatum leaves gradually decreased as the dose in mg / kg was increased. 

These results showed that although the OE of Piper marginatum leaves did not have a satisfactory effect in reducing glycemia in experimental models, it is believed that the results of this research contributed to the toxicological knowledge of the species studied, as it did not demonstrate toxicity when administered orally. 

Therefore, it is necessary to invest in more experimental studies with an in vitro approach in order to identify biological potential and complement the information presented in this study.

MEMBROS DA BANCA:
Externa ao Programa - 2375623 - GABRIELA BIANCHI DOS SANTOS
Presidente - 1661684 - KELLY CHRISTINA FERREIRA CASTRO
Externa à Instituição - LÍVIA TRINDADE LÔBO