Banca de DEFESA: KÁRITA JULIANA SOUSA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KÁRITA JULIANA SOUSA SILVA
DATE: 15/07/2022
TIME: 15:00
LOCAL: google meet
TITLE:

BEE Honey (Melipona scutellaris and Apis mellifera) IN THE TISSUE REPAIR OF INFECTED CUTANEOUS WOUNDS IN WISTAR RATS (rattus novergicus albinus)

KEY WORDS:

Honey; Healing; Wounds; Antimicrobian activity; Therapeutic action;

Amazon; Staphylococcus aureus.

PAGES: 72
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUMMARY:

In the last decades, studies began to analyze the therapeutic properties of honey, specifically in healing. Honey is a low-cost product, which facilitates access for the population. The objective of this research was to investigate the tissue repair process of skin wounds infected with Staphylococcus aureus in Wistar rats through the application of bee honey. It was a quantitative, analytical, experimental, controlled and randomized interventional study. The sample consisted of 30 male Wistar rats of the Rattus norvergicus albinus species, allocated into 3 groups of 10 animals each, divided into subgroups of 5 animals each: four experimental subgroups and two control subgroups. The groups had infected skin wounds with inoculation of 0.5 mL of suspension of a standard strain of Staphylococcus aureus (ATCC 25923), standardized in the laboratory according to the Mc Farland scale, at a concentration of 1.5x108 CFU/mL. The experimental group was subdivided for application to two types of honey: Melipona scutellaris, named for the study as (M01) and the other sample of Apis mellifera (A01). Physicochemical and biochemical analyzes and in vitro antimicrobial action were performed on the honeys for the selection of the two samples. Sterile swabs were collected from the lesions, and the material was seeded in Petri dishes to observe the infection induced by inoculating the suspension of S. aureus. After euthanasia, the tissues were stained with Picro-sirius red and Hematoxylin-eosin for histological analysis. In the antibacterial analysis, the results were satisfactory with a reduction in the reduction of bacteria in the plaques. In the macroscopic analysis, there was a reduction in the wound area in all groups, but the groups treated with honey showed significantly better differences in relation to the control group. In the evaluation of the mean intensity of the inflammatory infiltrate, there was a reduction in the infiltrate in all groups, but there was no significant difference. In the evaluation of collagen, the groups that received the treatment with Apis and Melipona honey showed a greater deposition of collagen fiber bundles, with a significant difference in relation to the control group. It is concluded that the groups treated with honey showed better results regarding the assessment of wound contraction area and collagen fiber deposition.

BANKING MEMBERS:
Presidente - 1770373 - PAULO SERGIO TAUBE JUNIOR
Interna - 1967452 - ADRIANA CAROPREZO MORINI
Externo à Instituição - JUAREZ DE SOUZA - UEPA
Externo à Instituição - HUGO DE CAMPOS BRAGA - UNIFESP