Banca de DEFESA: MIKE MENEZES PINTO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MIKE MENEZES PINTO
DATE: 28/04/2023
TIME: 14:00
LOCAL: Auditório do NTB
TITLE:

Metabolic profile evaluation of cattle and buffaloes at feedlot submitted to different diets

 

KEY WORDS:

 

PAGES: 58
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The metabolic profile can be used to monitor the metabolism adaptation to different types of diets, to diagnose possible nutrient homeostasis imbalances, and can also be used to identify causes of the manifestation of nutritional or metabolic diseases. The present study aimed to evaluate the organic and performance alterations of confined cattle and buffaloes that received different diets. A total of 24 crossbreed animals, 12 cattle and 12 buffaloes, aged 14-16 months and 200-300 kg body weight were used. The animals were housed in individual stalls with feeder and water supply and were subjected to a 15-day diet adaptation period. The animals were blocked according to weight in a 2x2 factorial arrangement, comparing animal species (buffalo and bovine) and within each species comparing two diets, one group receiving 100% whole corn diet (whole grain diet) and another group receiving 70% whole grain concentrate and 30% corn silage based on dry matter (conventional diet). Buffaloes and cattle were matched according to weight and randomly allocated to one of the 64-day dietary treatments in a randomized block design with time-repeated measures. The animals were weighed weekly after 14 hours of water and food fasting until the end of the experiment on day 64. Blood samples were obtained on the days of the weekly evaluations (D0, D7, D21, D35, D49, D64) by a jugular puncture. and placed in tubes with and if anticoagulant. Anticoagulant tubes were kept refrigerated immediately after collection until processing (maximum 6 hours), while tubes without anticoagulant were kept at room temperature, centrifuged at 1,000 x g for 10 minutes to obtain serum. Biochemical analyses were performed on a Daytona RX automated biochemistry analyzer at USP - University of São Paulo facilities at the FMVZ (Faculty of Veterinary Medicine and Biochemistry) laboratory to measure Total Protein, Albumin, Urea, Creatinine, Cholesterol concentrations. Triglycerides, Beta-hydroxybutyrate, NEFA, Total Bilirubin, Direct Bilirubin, Glucose, Lactate, Ca, P and Mg as well as enzyme activities AST, GGT, CK. Data were subjected to a distribution analysis and if they were normally distributed and compared by analysis of variance and Tukey test to evaluate the difference in daily weight gain at the end of the study. Biochemical variables evaluated by two-way repeated-measures ANOVA considering the experimental group and time factors followed by Bonferroni test. Data with non-normal distribution were compared between groups by the Kruskal-Wallis test and between moments by Friedman test between experimental times.

COMMITTEE MEMBERS:
Presidente - 1843993 - ANTONIO HUMBERTO HAMAD MINERVINO
Interno - 2383549 - GUSTAVO DA SILVA CLAUDIANO
Externo à Instituição - FÁBIO EDIR AMARAL ALBUQUERQUE