Banca de QUALIFICAÇÃO: ANDREZA DA SILVA PEIXOTO

CHARACTERIZATION OF THE PROFILE OF RESISTANCE TO ANTIMICROBIALS AND PROSPECTING THE ENZYME POTENTIAL OF ENVIRONMENTAL BACTERIA

Resistance. Antimicrobials. Enzymatic potential. Hydrocarbons

In addition to antimicrobial resistance, microorganisms are also widely tested for their ability to produce enzymes, being one of the most important sources of raw material used in biotechnological processes. The objective of this study will be to characterize the profile of resistance to antimicrobials and evaluate the potential in the production of enzymes of Corynebacterium spp. and Clostridium spp. For this, strains of both genders from environmental sources of different matrices will be used: stingrays (Chondrichthyes: Potamotrygoninae), soil with and without manipueira, basin and microbasin water from the Santarém region, and macrophyte (Eichhornia spp.). The strains from these matrices will be chosen at random. After selection, the strains will be identified by molecular method, with PCR analysis and DNA extraction. Subsequently, antimicrobial resistance profile tests will be performed by the Kirby-Bauer method using the disc diffusion technique. For the enzymatic potential tests, the production of four types of enzymes will be verified: amylase, cellulase, ligninase and protease, through specific means. Still continuing with the tests, the potential of the strains to degrade hydrocarbons through the Sabourad-Petróleo Agar medium will be evaluated. The antagonistic activity and determination of the Minimum Inhibitory Concentration (MIC) of bacterial extracts Corynebacterium spp. and Clostridium spp. by plate microdilution method. The expected results regarding the bacterial resistance profile, especially for the Corynebacterium spp. and Clostridium spp. where there are relatively few studies on species from environmental sources, it is expected to find different resistance profiles between the samples, given that they come from different substrates. Regarding the production of enzymes, degradation of hydrocarbons and antagonistic activity by environmental bacteria, given that these bacteria come from the Amazon region, which is an important source of discoveries for the scientific community, it is expected to find promising results in the objectives to be be studied.