Banca de QUALIFICAÇÃO: ANA CLAUDIA SOUZA ABREU

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA CLAUDIA SOUZA ABREU
DATE: 24/07/2023
TIME: 14:00
LOCAL: Sala 101B do NSA
TITLE:

SILVER NANOPARTICLES (AgNPs) SYNTHESIZED THROUGH STINGLESS BEE HONEY IN THE TISSUE REPAIR OF INFECTED CUTANEOUS WOUNDS: A PRECLINICAL STUDY

KEY WORDS:

Green synthesis. Wound healing. Honey. antimicrobial. Silver nanoparticles. Toxicity.

PAGES: 61
BIG AREA: Ciências Biológicas
AREA: Farmacologia
SUMMARY:

Infected wounds are increasingly a daily reality, when the healing process is complicated, it causes damage to the individual's health. Stingless bee honey (ASF) contains phenolic compounds, sugar and others in its composition, which promote various therapeutic purposes. The green synthesis of silver nanoparticles (AgNPs) has been developed because it is fast, cheap and ecological. The objective of this research is to evaluate the tissue repair process of cutaneous wounds infected with Staphylococcus aureus in an in vivo test, through the application of silver nanoparticles synthesized from ASF honey. 35 samples of ASF honey from rural producers in the state of Amazonas were donated. These samples underwent physical-chemical and biochemical analyses. In addition, the antimicrobial activity was evaluated by means of diffusion assay in wells and Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, and from the 35 samples, only two samples named JUP1 and JUP2 were selected from bee species Melipona compressipes manaosensis, which had the highest antimicrobial activities for performing AgNPs synthesis. AgNPs were characterized using Dynamic Light Scattering (DLS), surface Zeta Potential, stability of AgNPs over time and transmission electron microscopy (TEM). Subsequently, the toxicity test of the AgNPs synthesized through the JUP1 and JUP2 samples will be carried out, through the tests of acute oral toxicity and acute dermal toxicity in vivo. In addition, an in vitro eye irritation toxicity test will be performed. After carrying out the biosafety tests, the in vivo healing test will be started, which will have 60 rats of the Rattusnovergicus albinus species, which will have cutaneous wounds infected with Staphylococcus aureus, they will be drawn and distributed into 6 groups of 10 animals, thus named: GMJUP1 and GMJUP2 (treatment with Melipona compressipes manaosensis honey); GNJUP1 and GNJUP2 (treatment with AgNPs synthesized from Melipona compressipes manaosensis honey); GCS (treatment with 0.9% sodium chloride saline solution, characterized as the negative control); GCP (treatment with Neomycin Sulfate ointment, characterized as a positive control). Macroscopic and microscopic analyzes of the wounds will be made according to the date of euthanasia of the animals. Preliminary results on the physical-chemical and biochemical analysis of the honeys obtained variations between the samples, since the botanical origin, storage conditions, geographic location and others, influence the quality of the honeys. The synthesis of AgNPs from the JUP1 and JUP2 samples were verified with amber staining at concentrations of 3 mM and 5 mM at 80ºC. The characterization of the AgNPs showed that the values of DLS, Zeta Potential and morphology (spherical) were similar to each other. For the expected results, it is intended that the toxicity test can promote adequate biosafety in the healing test. It is expected that the experimental groups treated with AgNPs synthesized from ASF honeys may present the best results in tissue repair compared to the other groups.

COMMITTEE MEMBERS:
Presidente - 1770373 - PAULO SERGIO TAUBE JUNIOR
Interna - 1334145 - GRACIENE DO SOCORRO TAVEIRA FERNANDES
Externo à Instituição - JUAREZ DE SOUZA - UEPA