Banca de QUALIFICAÇÃO: SANTANA PINTO DE CASTRO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : SANTANA PINTO DE CASTRO
DATE: 28/07/2023
TIME: 08:30
LOCAL: Sala Multimeios -Proppit
TITLE:

CHEMICAL STUDY, MICROBIOLOGICAL AND ANTIOXIDANT ACTIVITIES OF ETHANOLIC EXTRACTS OBTAINED FROM ENDOPHYTICS FUNGI ISOLATED FROM Piper marginatum Jacq.

KEY WORDS:

Endophytics fungi, Piperaceae, Bioprospecting

PAGES: 35
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Bioquímica dos Microorganismos
SUMMARY:

The Piperaceae family widely used as food, condiment, ornamental plants and insecticide is known for the production of natural substances that have several medicinal properties reported in the literature. Widely found in tropical regions and composed of 5 genera: Zippelia, Verhuellia, Manekia, Peperomia and Piper. The genus Piper is one of the most abundant of this family, highlighting in this genus the species Piper Marginatum Jacq, being one of the main properties attributed to this species, its potential as a source of valuable antimicrobial compounds for direct use or in combination with other antimicrobials. Endophytic fungi are microorganisms that live inside the tissues of plants without causing negative effects having a mutual relationship with the host, in this case the plants and act protecting them against pathogens and obtaining nutrients for their survival. Through interaction with plants, endophytes produce several metabolites with pharmaceutical application, which has been used in the production of antimicrobials and inhibitors of various diseases. Several studies have shown that endophytic fungi are potential producers of a vast amount of important bioactive secondary metabolites, previously found only in plants. Despite few studies on the metabolites produced by endophytic fungi, there are already reports of a considerable number of substances with biological activity. Combined with the results already reported by our group on the species P. marginatum and few reports on the metabolic diversity of fungi associated with the species, this project aims to chemically characterize ethanolic extracts obtained from three species of endophytic fungi isolated from P. marginatum Jacq. from the municipality of Santarém-PA and to evaluate their antioxidant and antimicrobial activity. The endophytic fungi isolated from the leaves of P. marginatum will be provided in partnership with another dissertation project of the Graduate Program in Biosciences of the University Federal of Western Pará (UFOPA), which will carry out the isolation and genetic identification studies of these fungi. Initially the fungi will be incubated in semi-solid potato-dextrose-agar (BDA) culture medium for 7 days and then preserved in mineral oil to avoid dehydration. The preserved fungal cultures will serve as matrices for all the studies carried out in the work. Obtaining the fungal extracts will follow the methodologies adapted from Luiz (2021), in which the preserved cultures of the endophytes will initially be grown in Petri dishes containing 20 mL of potato-dextrose-agar (BDA) and, after seven days of incubation, discs of mycelia (5 mm) will be removed, containing their structures, and then added in solid culture medium of parboiled rice and extracted with ethyl alcohol P.A.. To obtain the crude extracts of endophytic fungi, after 25 days of incubation in the solid medium of parboiled rice, 100 ml of absolute ethyl alcohol P.A. 99.9% will be added to each bottle, and extraction by the maceration technique for a period of 24h, being filtered on qualitative filter paper and adding another 100 ml of solvent and repeating the process for another 24h, completing 48h of extraction by maceration. After the extraction, the extractive solutions will be filtered again and the solvent used in the extraction will be removed with the aid of a rotary evaporator under reduced pressure at 40 °C. The crude extracts obtained will be analyzed by Thin Layer Chromatography (TLC) for the detection of the following classes of metabolites namely, simple coumarins, glycosylated coumarins, terpenoids, fatty acids, flavonoids, condensed tannins and hydrolysable tannins according to the methodology of Wagner and Bladt (2001). Tests for determination of total phenolics will follow the methodology adapted from Singleton and Rossi (1965) and tests for determination of flavonoids will follow the methodology adapted from Woisky and Salatino (1998). For determination of antioxidant activity in chromatoplate will be used the method adapted from Ciesla et al., (2012) and antioxidant activity will be determined by the method described by Scherer and Godoy (2009). The antifungal activity will be performed by the bioautography technique for fungitoxic activity, by nebulizing the pathogenic fungi previously selected on CCD plates containing 200 μg of the crude extracts of fungi, previously eluted with the selected mobile phase. The plates will be incubated at 25º C for 48 hours, verifying the presence of halos of inhibition of the growth of the fungi, compared to the positive standard nystatin (10 μg). It is intended from the results found to prospect as promising sources of bioactive secondary metabolites, the endophytic fungi associated with the medicinal species P. marginatum, and should later be studied for the development of new drugs from the classes of metabolites that will be found.

COMMITTEE MEMBERS:
Presidente - 1661684 - KELLY CHRISTINA FERREIRA CASTRO
Interno - 1770373 - PAULO SERGIO TAUBE JUNIOR
Externo ao Programa - 1574066 - ALCICLEY DA SILVA ABREU - UFOPA