PHYTOCHEMICAL CHARACTERIZATION, EVALUATION OF THE ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL THROUGH SILVER NANOPARTICLES FROM THE PLANT SPECIES Dipteryx odorata
antioxidant activity; coumaru; AgNPs; antimicrobial; green synthesis
Since the discovery of penicillin in 1928, the industry has been developing several studies in the search for new treatments for the most diverse types of bacteria, likewise, considering the growth of the Brazilian and world population, the search for a change in lifestyle and the The search for more quality of life has made the population invest in health, the use of products with antioxidant activity has gained prominence, since the effects of these products can be very beneficial for health. It is known that 80% use medicinal plants to treat some type of illness, mainly for the less favored, marginalized and low-income population. Cumaru or Dipterix odorata is a plant species, widely used in the extractive industry, because it has a market potential due to its particularities, this has aroused the interest for research in the area of biotechnology and pharmaceuticals, in view of which we can list the primordial role of nanotechnology Allied to the therapeutic potential of Cumaru, nanotechnology is a cutting-edge area with numerous industrial applications and has opened new frontiers applied in biotechnology, biosciences, materials science and engineering. Green synthesis, a method that has been widely used to generate metallic nanoparticles and, depending on the metallic structure, some organic compounds are suitable for the formation of nanostructures. AgNPs are evidenced and can be used to reduce infections, prevent bacterial colonization on prosthetic surfaces, in catheters and dental materials, as well as in the food industry, in water treatment and in the manufacture of antibacterial paints. Therefore, this project aims to identify secondary metabolites present in the essential oil and extracts of dipteryx odorata that have antioxidant and antimicrobial action, from silver nanoparticles. To carry out the experiment, the extraction of the essential oil by cold pressing will be carried out, and the alcoholic and aqueous extract by means of cold extraction. For the analysis of secondary metabolites, gas chromatography and mass spectrometry will be used, with regard to antioxidant capacity, the analysis will be done using DPPH and iron reducing power and ABTS. After analyzing the components, the essential oil and extracts will be tested in vitro, using petri dishes, some species of bacteria considered resistant to antibiotics, the experiments will be carried out in triplicate and will have a blank as molecular weight or control. Therefore, it is expected to be able to identify the main secondary metabolites present in the essential oil of the species Dipteryx odorata, in addition to identifying which of these metabolites are responsible for the antimicrobial and antioxidant actions.