Banca de DEFESA: DAIANE SILVA RODRIGUES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DAIANE SILVA RODRIGUES
DATE: 19/03/2024
TIME: 14:00
LOCAL: Santarém-PA
TITLE:

FUNGAL DIVERSITY WITH EMPHASIS ON THE BOTRYOSPHAERIACEAE FAMILY ASSOCIATED WITH CASSAVA STEM CUTTINGS

KEY WORDS:

Phytopathology. Etiology. Dominant marker. Ascomycetes.

PAGES: 58
BIG AREA: Ciências Biológicas
AREA: Biotecnologia
SUBÁREA: Biotecnologia Vegetal
SPECIALTY: Melhoramento de Plantas
SUMMARY:

Cassava (Manihot esculenta Crantz) is considered of great importance in food security, with a tuberous root, rich in starch, and with high caloric value. Root and stem rot are diseases that compromise the crop, causing major losses, through direct losses in production and through the spread of pathogens, respectively. Studies have revealed the occurrence of a diversity of phytopathogenic fungi, particularly those from the Botryosphaeriaceae family, associated with stem rot. This work aimed to identify fungal diversity with emphasis on the Botryosphaeriaceae family, associated with cassava stem cuttings, after disinfection and induction of rot. Fungal isolations were carried out by serial dilution, of the rot stem from municipalities of Alenquer, Belterra, Curuá, Faro, Itaituba, Juruti, Mojuí dos Campos, Prainha and Santarém, followed by DNA extraction using the Wizard® Genomic DNA Purification kit. (Promega). To study diversity, identification was carried out by amplification of the ITS region with the universal primers ITS1 and ITS4 and subsequent sequencing by Sanger method. The electropherograms were trimmed and edited so that the contigs could be used for identification. Molecular identification was initiated by using Blast and subsequent comparison with standard sequences of the species of the associated genus/section/complex. Phylogenetic analysis of species from the Botryosphaeriaceae family was carried out in the gene regions of the translation elongation factor (TEF 1 – α), RNA polymerase subunit II (rpb2) and internal transcribed spacer (ITS). Eleven genera were identified in the fungal diversity in stem cuttings (Lasiodiplodia, Neoscytalidium, Fusarium, Penicillium, Aspergillus, Cladosporium, Hyphopichia, Trichoderma, Clonostachys, Diaporthe and Phomopsis). The genus Lasiodiplodia was the most frequent, with several species detected, distributed differentially among the different communities and municipalities in the western region of Pará, and all isolates of Lasiodiplodia and Neoscytalidium were capable of causing lesions in cassava roots and were reisolated, confirming its pathogenicity. These data demonstrate the importance of healthy propagation material, as pathogens can be disseminated even when asymptomatic and with superficial disinfection treatments.

COMMITTEE MEMBERS:
Presidente - 1966924 - CARLOS IVAN AGUILAR VILDOSO
Interno - 2383549 - GUSTAVO DA SILVA CLAUDIANO
Externo ao Programa - 1793956 - MARCOS PRADO LIMA - UFOPAExterna à Instituição - ALESSANDRA KEIKO NAKASONE - EMBRAPA