Banca de DEFESA: ISABELE DE AZEVEDO PORTELA ALMEIDA

EVALUATION OF THE ANTIMICROBIAL EFFECT OF COPAIBA OIL FRACTIONS (Copaifera reticulata Ducke) AGAINST ACNE-CAUSING BACTERIA - Cutibacterium acnes ALLIED TO THE DEVELOPMENT OF COSMETIC FORMULATION

Keywords: Cutibacterium acnes. Copaiba. Acne. Formulation

Acne vulgaris is characterized as a multifactorial skin disease that results from the complex interaction between hormonal, inflammatory, genetic and environmental factors and presents skin lesions that can be inflammatory or non-inflammatory. The skin is composed of a microbiota with a variety of microorganisms including Cutibacterium acnes that can become opportunistic when sebum production increases due to the action of androgenic hormones. Among the variety of plant species are plants of the genus Copaifera, from which an oleoresin can be extracted and used as an anti-inflammatory, anti-ulcerogenic, bactericidal and in several other pathologies. Taking into account the importance of evidence confirming the benefits and efficacy of new alternative therapies in the treatment of acne using medicinal plants, the objective of this work is to evaluate the antimicrobial effect of the fractions (resinous and essential) of copaiba oil (Copaifera reticulata Ducke) against the bacteria that causes acne – Cutibacterium acnes and to develop a semi-solid cosmetic formulation. The bacterial species Cutibacterium acnes (ATCC 6919) will be purchased commercially in lyophilized form and transferred to plates containing defibrinated sheep blood agar. The antimicrobial activity will be initially analyzed using the disk diffusion method in petri dishes containing Blood Agar. For the Minimum Inhibitory Concentration (MIC), the bacterial inoculum will initially be adjusted to the standard 0.5 on the McFarland scale (1.5x108 CFU/mL), with subsequent dilution by a factor of 10, to obtain the working concentration of 1.5x104 CFU . It will be performed in 96-well microplates, filled with Thioglycolate broth at different concentrations. Subsequently, the Minimum Bactericidal Concentration (MBC) will be determined by transferring 10 µL of the wells corresponding to the MIC, and antecedents to the MIC, to plates containing defibrinated sheep blood agar and the development of a cosmetic formulation will be carried out and the antimicrobial activity evaluated by the technique of diffusion in wells. It is expected that the fractions of copaiba oleoresin present satisfactory antimicrobial activity against the bacteria that cause acne, becoming a complementary alternative in the treatment, reducing the adverse and unwanted effects caused by the medications used conventionally.