Banca de QUALIFICAÇÃO: LUDYANNE DA SILVA SOUSA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LUDYANNE DA SILVA SOUSA
DATE: 30/04/2024
TIME: 14:00
LOCAL: Universidade Federal do Oeste do Pará - Campus Tapajós
TITLE:

 

 

EFFECT OF CADMIUM AND COPPER ON THE GERMINATION AND INITIAL DEVELOPMENT OF Handroanthus serratifolius (VAHL) S.O. GROSE

KEY WORDS:

 

 

Heavy metals; LC50; Yellow Ipê; Mycorrhiza; Biotechnology.

PAGES: 38
BIG AREA: Ciências Biológicas
AREA: Ecologia
SUBÁREA: Ecologia Aplicada
SUMMARY:

 

 

The increase in human activities, such as agriculture and industry, has led to the accumulation of heavy metals in the soil, especially cadmium and copper, due to the widespread use of chemicals. This contamination harms the germination and initial growth of various plants, both cultivated and wild, causing effects such as inhibition of carbohydrate mobilization, disruption of osmoregulation, reduction in nutrient availability, deficiency in water absorption, retarded growth, and inhibition of root growth, chlorosis, and leaf necrosis. Toxicity tests are essential to assess the risks of this contamination in forest ecosystems. Handroanthus serratifolius is a common tree in Brazilian biomes, and the genus has shown varying degrees of sensitivity to heavy metals in previous studies. One way to mitigate the toxic effects of heavy metals is through association with arbuscular mycorrhizal fungi (AMF), which induce plant tolerance, alleviate oxidative stress caused by heavy metals, and alter root morphology. Therefore, this work divided into two bioassays, an acute toxicity test on seeds and a chronic toxicity test on seedlings. The first assay aims to evaluate the effect of cadmium, copper, and the mixture of both contaminants on the germination of H. serratifolius seeds. In the second assay, the objective is to evaluate the growth and physiological parameters of H. serratifolius seedlings subjected to different concentrations of cadmium, in the presence and absence of AMF. As an exposure marker in germination, the seeds will divide into 22 plots with 4 repetitions of 25 seeds, where the repetitions will sow in Gerbox-type boxes between Germitex papers, previously moistened with the contaminant solution. Cadmium sulfate, copper sulfate, and the mixture of contaminants will use as toxicants. As markers of toxic effect, the percentage of total germination, number of seedlings, germination speed index, and protoplasmic tolerance will analyze. In seedlings, exposure marker will be performed from the 4 x 2 factorial scheme consisting of 4 concentrations of cadmium in the soil with and without mycorrhiza. The markers of effect analyzed will assess by comparing the impact of different concentrations of cadmium on growth and the quantification of nitrogenous compounds concentration, gas exchanges, pigments, enzymatic and non-enzymatic antioxidant activities. Quantification of fungal structures will also evaluate as a means of comparing the impact of different concentrations of cadmium on mycorrhizal colonization. The 50% lethal concentration will be determined using the Trimmed Spearman-Karber test and the remaining analyzes will be subjected to the Shapiro-Wilk t
est (p>0.05) and means compared by Tukey's test at the 5% significance level.

COMMITTEE MEMBERS:
Presidente - 1836832 - RUY BESSA LOPES
Interno - 1770373 - PAULO SERGIO TAUBE JUNIOR
Externo ao Programa - 1962665 - ELCIO MEIRA DA FONSECA JUNIOR - UFOPA