Banca de DEFESA: GISELE FERREIRA DE SOUSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GISELE FERREIRA DE SOUSA
DATE: 30/06/2023
TIME: 14:30
LOCAL: Sala 110B NSA/Tapajós
TITLE:

REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION (RT-LAMP) ASSAY FOR THE DETECTION OF SARS-COV-2 INFECTION

KEY WORDS:

Covid-19, SARSC-CoV-2, LAMP

PAGES: 47
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

COVID-19, the SARS-CoV-2 pandemic in the world caused a large infected population manifesting itself mainly as a respiratory infection. Aiming to develop methodology for molecular diagnosis of COVID-19 by reverse transcription followed by loop-mediated isothermal amplification (RT-LAMP), allowing visual detection and rapid obtaining of the final result. To contain the spread of the virus, the WHO urgently required an extension of screening and testing; thus, a quick and simple diagnostic method is needed. However, studies conducted at several research centers indicate that laboratory results can provide clinical staff with many useful markers.Studies show the development of a molecular diagnostic methodology that allows detection of the virus through reverse transcription reaction followed by loop-mediated isothermal amplification (RT-LAMP). RT-LAMP is an important tool for the molecular diagnosis of infectious diseases, presenting great potential for point-of-care applications for both diagnostics and epidemiological studies, especially in developing countries. Research at the Federal University of Western Pará. This is a descriptive study with a quantitative approach, carried out through data collected in the database of the clinical analysis laboratory located in the city of Santarém-PA from December 2021 to January 2022. The study sample consisted of by individuals who obtained confirmation of Covid-19 infection by the RT-PCR methodology. Confirmed cases of Covid-19 were predominantly brown (83%) and female (53%) The predominant age group of patients was 40 to 49 years (28%). The most frequent clinical signs and symptoms were: cough (31.6%), fever (26.67%), headache (23.33%) and dyspnea (13.33%). After extraction, the samples were subjected to the LAMP test, which was carried out in a water bath with a defined temperature of 65°C, being observed after its reaction. RT-LAMP reactions were performed using two colorimetric LAMP kits and results were determined by visually assessing the color change of the indicator from dark pink to yellow. This work is of paramount importance, and in an innovative perspective of disseminating this methodology, for direct use in biological samples, under safe conditions through molecular techniques as a tool for diagnosis, which may favor the initiation of treatment as quickly as possible, thus avoiding complications of the disease.

COMMITTEE MEMBERS:
Externo à Instituição - CASSIANO JUNIOR SAATKAMP
Externa à Instituição - FRANCIANE DE PAULA FERNANDES - UEPA
Presidente - 1327077 - LUIS REGINALDO RIBEIRO RODRIGUES
Interna - ***.421.662-** - SANDRA LAYSE FERREIRA SARRAZIN - UFPA