Banca de QUALIFICAÇÃO: JULIANA MARIA DOS SANTOS RIBEIRO

IN VITRO STUDY OF THE PROBIOTIC ACTION OF LACTIC ACID BACTERIA ISOLATED FROM TARUBÁ ON SKIN CELLS

Ultraviolet Radiation, Microorganisms, Photoprotection

Chronic exposure to ultraviolet radiation (UVR) leads to several skin problems, such as
changes in the autochthonous microbiota, favoring the proliferation of pathogens and,
consequently, the emergence of dermal conditions; premature aging of the skin, through an
increase in free radicals, which damages collagen and elastin fibers; the dysregulation of the
immune system, keeping the human body constantly inflamed, stimulating the development,
in some cases, of skin neoplasms. For prevention, physical and/or chemical photoprotectors
are used, however, some components present in the composition cause harmful effects, due
to toxicity. Furthermore, sunscreens have the capacity to protect the passage of radiation to
the skin by up to 98%, which is why adjuvants are essential to maintain protection and avoid
disorders. A promising alternative is the use of probiotics, which are already being studied
for the prevention and treatment of harmful effects caused by unprotected exposure to UVR.
The objective of this project, therefore, is to evaluate potentially probiotic bacteria in
epithelial cells, aiming at skin health. To this end, nine bacteria isolated from Tarubá, a
fermented drink from the Amazon, with lactic acid characteristics, will be evaluated for their
antioxidant potential, through cell-free supernatant, and antimicrobial potential against skin
pathogens. After this, the bacteria with the best results will be tested for their cytotoxicity and
photoprotective and immunomodulatory capabilities in HaCat epithelial cells. The results are
expected to be lactic acid bacteria with preventive and therapeutic properties for skin
disorders caused by UVR. That have antioxidant potential and inhibit the growth of skin
pathogens, in addition to preventing the entry of UV into the skin and positively modulating
the immune system, so that in the future they can be used in the preparation of a
photoprotective p