Banca de QUALIFICAÇÃO: JACQUELINE PARENTE DE SOUSA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JACQUELINE PARENTE DE SOUSA
DATE: 25/04/2024
TIME: 15:00
LOCAL: Miniauditório Alter do Chão (BMT1- Sala 212)
TITLE:

ANTIOXIDANT ACTIVITY AND IN VITRO ENZYME INHIBITION OF ANDIROBA (Carapa guianensis aubl) INDUSTRIAL WASTE AND CUMARU (Dipteryx odorata) SEEDS.

KEY WORDS:

Digestive enzymes, Diabetes, Andiroba and Cumaru.

PAGES: 52
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Diabetes mellitus (DM) is a chronic disorder caused by the lack of insulin production by pancreatic beta (β) cells or by defects in insulin receptors on target cells, resulting in metabolic disease. There are two general types of diabetes mellitus: type 1, caused by the absence of insulin secretion, and type 2, marked by insulin resistance. Therefore, bioprospecting for therapeutic alternatives, especially those of plant origin, is a promising strategy for discovering new drugs. Among the medicinal plants used in the Amazon is andiroba (Carapa guianenses Aubl), studies report its popular use for treating fever and rheumatism, anti-allergic, analgesic, chemotherapy and anti-inflammatory effects. Another plant widely used in traditional medicine is Dipteryx odorata, known as cumaru, which has antioxidant, anti-inflammatory and enzyme inhibitory effects. The objective of the work is to evaluate the antioxidant potential, the inhibitory capacity against the enzymes α-glucosidase and lipase and to determine the content of total phenols and flavonoids in andiroba resin oil and cumaru seed oil in in vitro experimental models. The industrial andiroba residue was obtained from amazonOIL, and the cumaru seeds were obtained from the EMATER farm located in Mojuí dos Campos. The oil from cumaru seeds will be carried out through hydroalcoholic extraction using the Soxhlet method, the extraction will last for 48 uninterrupted hours, until the sample is exhausted. To carry out the tests, both will be (1mg/ml) in 2% dimethyl sulfoxide. The antioxidant activity will be determined by evaluating the capacity to scavenge the radicals DPPH• (1,1-diphenyl-2-picrylhydrazyl) and ABTS•+ [2,2'-azino-bis acid (3-ethylbenzothiazoline-6- sulfonic). To evaluate enzymatic inhibition, photocolorimetric methods based on the release of ρ-nitrophenol will be used. The total phenolic content will be determined by the Folin-Ciocalteu method and the total flavonoid content will be evaluated based on the reaction with 10% aluminum chloride and 1.0 M potassium acetate. All tests will be carried out in triplicate.

COMMITTEE MEMBERS:
Presidente - 1834385 - WALDINEY PIRES MORAES
Interna - 3304272 - RAYANNE ROCHA PEREIRA
Externo ao Programa - 3390915 - ANDREI SILVA FREITAS - UFOPA