Banca de DEFESA: ALINE DE MORAIS GOMES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ALINE DE MORAIS GOMES
DATE: 25/06/2024
TIME: 15:00
LOCAL: Miniauditório Alter do Chão (BMT1- Sala 212)
TITLE:

EVALUATION OF THE IN VITRO AND IN VIVO ANTIDIABETTIC ACTIVITY OF THE EXTRACT OF Microdesmia rigida (BENTH)

KEY WORDS:

Oiticica. Medicinal plants. Diabetes Mellitus. Obesity. Antioxidant activity.

PAGES: 62
BIG AREA: Ciências Biológicas
AREA: Farmacologia
SUMMARY:

Diabetes mellitus is a metabolic disorder characterized by high blood glucose levels. Despite the treatments available on the market, controlling the complications of the disease is still a challenge. Given the severity of diabetes, it is necessary to search for alternative and innovative measures for its regulation. Medicinal plants with their preventive and curative properties become an alternative in this search. Therefore, the objective of this work was to evaluate the in vitro and in vivo antidiabetic activity of Microdesmia rigida (Benth.) extract, one of the plants used in folk medicine as an antidiabetic agent. The aqueous extract of M. rigida (EAMr) was obtained from the collection and drying of leaves obtained in the municipality of Santarém, Pará, then ground and decocted and lyophilized. Chemical characterization was carried out by Liquid Chromatography Coupled to the Diode Array Detector and Mass Spectrometry with an electrospray ionization source. The quantification of phenolic content was determined by the Folin-Ciocalteu method and flavonoids occurred from the reaction with 10% aluminum chloride and 1.0 M potassium acetate. Antioxidant activity was defined by evaluating the inhibition capacity against free radical DPPH• (1,1-diphenyl-2-picrylhydrazyl) and ABTS•+ [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)]. Photocolorimetric methods based on the release of ρ-nitrophenol were used to evaluate the inhibition capacity on the enzymes α-glucosidase and lipase. The assessment of acute oral toxicity in Swiss mice was carried out as recommended in the OECD/OECD Guide 423 (2001) and the hypoglycemic activity was analyzed in diabetic Swiss mice, induced with alloxan and treated with EAMr at doses of 100, 200 and 400mg/ kg, administered by gavage for 20 days. Blood glucose and physiological parameters were measured on the 1st, 10th and 20th day of treatment. The major presence of the Mirictrin compound was evident. EAMr presented total phenolic content (65.15±6.59 mg EAG/g) and flavonoids (24.57±2.64 mg EQ/g) and MIC50 of 17.23±1.18 µg/ml in the DPPH• assay and MIC50 of 11.95±0.52 µg/ml in the ABTS•+ assay. The EAMr showed a percentage of enzyme inhibition lower than 35%. The acute toxic dose of EAMr is greater than 2000 mg/kg in Swiss mice. In vivo, the glucose level was significant at the 400mg/kg dose when compared with the diabetic control group. Other experimental studies with a different approach are necessary to complement the information presented in this study.

COMMITTEE MEMBERS:
Presidente - 1834385 - WALDINEY PIRES MORAES
Interno - 1843993 - ANTONIO HUMBERTO HAMAD MINERVINO
Externo ao Programa - 3390915 - ANDREI SILVA FREITAS - UFOPAExterno à Instituição - JOSE SOUSA DE ALMEIDA JUNIOR - UFOPA